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Compounds were fully characterized by various spectroscopic and analytical techniques. The molecular structures of four derivatives were confirmed by X\u2010ray crystallography. The stability of the ligands and the complexes in aqueous medium was monitored spectroscopically. Both the ligands and the complexes were shown to interact with calf thymus DNA (ct\u2010DNA). Additionally, structures containing a phenol pendant arm were significantly more cytotoxic than those carrying a pendant pyridine substituent, reaching sub\u2010micromolar IC<jats:sub>50<\/jats:sub> values on the triple\u2010negative human breast cancer cell line MDA\u2010MB\u2010231. The metal chelation and transchelation ability of the compounds towards Fe<jats:sup>II<\/jats:sup>, Fe<jats:sup>III<\/jats:sup> and Zn<jats:sup>II<\/jats:sup> ions was explored as a possible mechanism of action of these compounds.<\/jats:p>","DOI":"10.1002\/cbic.202000122","type":"journal-article","created":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T08:55:21Z","timestamp":1586768121000},"page":"2474-2486","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Isoxazole\u2010Derived Aroylhydrazones and Their Dinuclear Copper(II) Complexes Show Antiproliferative Activity on Breast Cancer Cells with a Potentially Alternative Mechanism Of Action"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4344-2469","authenticated-orcid":false,"given":"Jesica Paola","family":"Rada","sequence":"first","affiliation":[{"name":"LABSO-Bio Laboratory, Department of Chemistry Pontifical Catholic 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