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For all conjugate organometallic unit\/coumarins, an almost complete loss of fluorescence efficacy was observed. However, the nature of the fluorophore, the type of bonding, the presence and length of a linker between the coumarin dye and the ruthenium(II) moiety, and the number of dye units influenced their biological properties. The <jats:italic>in\u2005vitro<\/jats:italic> activity against a transgenic <jats:italic>T. gondii<\/jats:italic> strain grown in human foreskin fibroblasts (HFF) leads to IC<jats:sub>50<\/jats:sub> values for <jats:italic>T. gondii<\/jats:italic> \u03b2\u2010gal from 105 to 735\u2005nM. Of note is that nine compounds displayed lower IC<jats:sub>50<\/jats:sub> than the standard drug pyrimethamine. One compound applied at its IC<jats:sub>50<\/jats:sub> did not affect B\u2010cell proliferation but had an impact on T\u2010cell proliferation in murine splenocyte cultures. Transmission electron microscopy of <jats:italic>T. gondii<\/jats:italic> \u03b2\u2010gal\u2010infected HFF showed that treatment predominantly affected the parasites\u2019 mitochondrion.<\/jats:p>","DOI":"10.1002\/cbic.202000174","type":"journal-article","created":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T05:36:44Z","timestamp":1588138604000},"page":"2818-2835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Coumarin\u2010Tagged Dinuclear Trithiolato\u2010Bridged Ruthenium(II)\u22c5Arene Complexes: Photophysical Properties and Antiparasitic Activity"],"prefix":"10.1002","volume":"21","author":[{"given":"Oksana","family":"Desiatkina","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Bern  Freiestrasse 3 3012 Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"P\u0103unescu","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Bern  Freiestrasse 3 3012 Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"M\u00f6sching","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Bern  Freiestrasse 3 3012 Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicoleta","family":"Anghel","sequence":"additional","affiliation":[{"name":"Institute of Parasitology Vetsuisse Faculty University of Bern  L\u00e4nggass-Strasse 122 3012 Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghalia","family":"Boubaker","sequence":"additional","affiliation":[{"name":"Institute of Parasitology Vetsuisse Faculty University of Bern  L\u00e4nggass-Strasse 122 3012 Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosra","family":"Amdouni","sequence":"additional","affiliation":[{"name":"Institute of Parasitology Vetsuisse Faculty University of Bern  L\u00e4nggass-Strasse 122 3012 Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0622-2128","authenticated-orcid":false,"given":"Andrew","family":"Hemphill","sequence":"additional","affiliation":[{"name":"Institute of Parasitology Vetsuisse Faculty University of Bern  L\u00e4nggass-Strasse 122 3012 Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2096-0618","authenticated-orcid":false,"given":"Julien","family":"Furrer","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Bern  Freiestrasse 3 3012 Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,16]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b01689"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CS00332C"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24101995"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/inorganics7030031"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","unstructured":"\u201cRuthenium Anticancer Agents: From Cisplatin Analogues to Rational Drug Design\u201d M. 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