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In this study, we aimed to develop a new photocatalytic hydrogen\u2010production system based on <jats:italic>Escherichia coli<\/jats:italic> whole\u2010cell genetically engineered as a biocatalysis for highly active hydrogen formation. The photocatalysis part was obtained by bacterial precipitation of cadmium sulfide (CdS), which is a visible\u2010light\u2010responsive semiconductor. The recombinant <jats:italic>E. coli<\/jats:italic> cells were sequentially subjected to CdS precipitation and heterologous [FeFe]\u2010hydrogenase synthesis to yield a CdS@<jats:italic>E. coli<\/jats:italic> hybrid capable of light energy conversion and hydrogen formation in a single cell. The CdS@<jats:italic>E. coli<\/jats:italic> hybrid achieved photocatalytic hydrogen production with a sacrificial electron donor, thus demonstrating the feasibility of our system and expanding the current knowledge of photosensitization using a whole\u2010cell biocatalyst with a bacterially precipitated semiconductor.<\/jats:p>","DOI":"10.1002\/cbic.202000383","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T09:13:30Z","timestamp":1595409210000},"page":"3389-3397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Photo\u2010biohydrogen Production by Photosensitization with Biologically Precipitated Cadmium Sulfide in Hydrogen\u2010Forming Recombinant <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6342-1924","authenticated-orcid":false,"given":"Yuki","family":"Honda","sequence":"first","affiliation":[{"name":"Department of Chemistry, Biology, and Environmental Science Faculty of Science Nara Women's University  Kitauoyanishi-machi Nara 630-8506 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Shinohara","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biology, and Environmental Science Faculty of Science Nara Women's University  Kitauoyanishi-machi Nara 630-8506 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motonori","family":"Watanabe","sequence":"additional","affiliation":[{"name":"International Institute for Carbon-Neutral Energy Research Kyushu University  744 Motooka, Nishi-ku Fukuoka 819-0395 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsumi","family":"Ishihara","sequence":"additional","affiliation":[{"name":"International Institute for Carbon-Neutral Energy Research Kyushu University  744 Motooka, Nishi-ku Fukuoka 819-0395 Japan"},{"name":"Department of Applied Chemistry Faculty of Engineering Kyushu University  744 Motooka, Nishi-ku Fukuoka 819-0395 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biology, and Environmental Science Faculty of Science Nara Women's University  Kitauoyanishi-machi Nara 630-8506 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,31]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rser.2015.12.112"},{"key":"e_1_2_7_2_1","unstructured":"\u00a0"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-018-0251-7"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotochemrev.2019.04.002"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-020-0428-y"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotochemrev.2017.09.001"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201710070"},{"key":"e_1_2_7_7_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201710070"},{"key":"e_1_2_7_8_1","unstructured":"\u00a0"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja101031r"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja2116348"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja413001p"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4CP05993J"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1039\/B817371K"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja907923r"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8EE02361A"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr4005814"},{"key":"e_1_2_7_17_1","unstructured":"\u00a0"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b02549"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat9777"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-018-0267-z"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)81197-3"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1381-1169(99)00417-3"},{"key":"e_1_2_7_23_1","unstructured":"\u00a0"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201600177"},{"key":"e_1_2_7_24_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201600177"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apcatb.2017.04.015"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CY00128G"},{"key":"e_1_2_7_27_1","author":"Honda Y.","year":"2020","journal-title":"Catal. 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