{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:35:57Z","timestamp":1786977357250,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T00:00:00Z","timestamp":1600905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006512","name":"School of Medicine, Duke University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006512","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2021,1,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ramoplanins and enduracidins are peptidoglycan lipid intermediate II\u2010binding lipodepsipeptides with broad\u2010spectrum activity against methicillin\u2010 and vancomycin\u2010resistant Gram\u2010positive pathogens. Targeted genome mining using probes from conserved sequences within the ramoplanin\/enduracidin biosynthetic gene clusters (BGCs) was used to identify six microorganisms with BGCs predicted to produce unique lipodepsipeptide congeners of ramoplanin and enduracidin. Fermentation of\n                    <jats:italic>Micromonospora chersina<\/jats:italic>\n                    yielded a novel lipoglycodepsipeptide, called chersinamycin, which exhibited good antibiotic activity against Gram\u2010positive bacteria (1\u20132\u2005\u03bcg\/mL) similar to the ramoplanins and enduracidins. The covalent structure of chersinamycin was determined by NMR spectroscopy and tandem mass spectrometry in conjunction with chemical degradation studies. These six new BGCs and isolation of a new antimicrobial peptide provide much\u2010needed tools to investigate the fundamental aspects of lipodepsipeptide biosynthesis and to facilitate efforts to produce novel antibiotics capable of combating antibiotic\u2010resistant infections.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202000555","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T21:27:27Z","timestamp":1598563647000},"page":"176-185","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Discovery of Six Ramoplanin Family Gene Clusters and the Lipoglycodepsipeptide Chersinamycin**"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2530-015X","authenticated-orcid":false,"given":"Kelsey T.","family":"Morgan","sequence":"first","affiliation":[{"name":"Department of Chemistry Duke University  Durham NC 27708 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Chemistry Duke University  Durham NC 27708 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3253-9233","authenticated-orcid":false,"given":"Dewey G.","family":"McCafferty","sequence":"additional","affiliation":[{"name":"Department of Chemistry Duke University  Durham NC 27708 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,24]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1266"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.37.309"},{"key":"e_1_2_7_3_1","first-page":"451","volume":"16","author":"Parenti F.","year":"1990","journal-title":"Drugs Exp. Clin. Res."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.21.126"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.10296"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/B515328J"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr030106n"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00061-14"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.2.385"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg274"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.37.318"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm070042z"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.21.119"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.21.147"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.3.1157-1159.2005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00096"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0000000000000332"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04853-14"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6NP00025H"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.1606"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904686106"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja011080p"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi961017q"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja035217i"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja000182x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja039671y"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja047879t"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2009.09.001"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja068573k"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0212314"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)81769-V"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401419101"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000095"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-013-1233-3"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-7014-2"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/np900710q"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0154121"},{"key":"e_1_2_7_38_1","unstructured":"R. Ciabatti B. Cavalleri European Patent Number EP0337203B11989."},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2015.04.015"},{"key":"e_1_2_7_40_1","first-page":"606","volume":"3","author":"McCormick M. H.","year":"1955","journal-title":"Antibiot. Annu."},{"key":"e_1_2_7_41_1","first-page":"e00296","volume":"3","author":"Lei X.","year":"2015","journal-title":"Genome"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1078\/072320203322337290"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.44.1300"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-019-0400-9"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(00)00091-0"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(99)80082-9"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-7-78"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106580200"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221582098"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-013-1261-z"},{"key":"e_1_2_7_51_1","unstructured":"I. Nogami H. Shirafuji S. Matsumura United States Patent Office Canada Patent Number CA1187016A1984."},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01583725"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19990305)62:5<576::AID-BIT9>3.0.CO;2-P"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-1139-x"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2876"},{"key":"e_1_2_7_56_1","first-page":"343","volume":"8","author":"Cruz M.","year":"2017","journal-title":"Front. Matrix Biol."},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90348-5"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202000555","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbic.202000555","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202000555","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T09:20:07Z","timestamp":1760260807000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.202000555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,24]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1,5]]}},"alternative-id":["10.1002\/cbic.202000555"],"URL":"https:\/\/doi.org\/10.1002\/cbic.202000555","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,24]]},"assertion":[{"value":"2020-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}