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In this work, we demonstrate that a rather unexplored class of enzymes can also be used for this. We used an F<jats:sub>420<\/jats:sub>\u2010dependent alcohol dehydrogenase (ADF) from <jats:italic>Methanoculleus thermophilicus<\/jats:italic> that was found to reduce various ketones to enantiopure alcohols. The respective (<jats:italic>S<\/jats:italic>) alcohols were obtained in excellent enantiopurity (&gt;99\u2009% <jats:italic>ee<\/jats:italic>). Furthermore, we discovered that the deazaflavoenzyme can be used as a self\u2010sufficient system by merely using a sacrificial cosubstrate (isopropanol) and a catalytic amount of cofactor F<jats:sub>420<\/jats:sub> or the unnatural cofactor FOP to achieve full conversion. This study reveals that deazaflavoenzymes complement the biocatalytic toolbox for enantioselective ketone reductions.<\/jats:p>","DOI":"10.1002\/cbic.202000651","type":"journal-article","created":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T16:52:38Z","timestamp":1600275158000},"page":"156-159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Facile Stereoselective Reduction of Prochiral Ketones by using an F<sub>420<\/sub>\u2010dependent Alcohol Dehydrogenase"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3710-2177","authenticated-orcid":false,"given":"Caterina","family":"Martin","sequence":"first","affiliation":[{"name":"Molecular Enzymology Group University of Groningen  Nijenborgh 4 Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5174-913X","authenticated-orcid":false,"given":"Gwen","family":"Tjallinks","sequence":"additional","affiliation":[{"name":"Molecular Enzymology Group University of Groningen  Nijenborgh 4 Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7264-1630","authenticated-orcid":false,"given":"Milos","family":"Trajkovic","sequence":"additional","affiliation":[{"name":"Molecular Enzymology Group University of Groningen  Nijenborgh 4 Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6346-5014","authenticated-orcid":false,"given":"Marco W.","family":"Fraaije","sequence":"additional","affiliation":[{"name":"Molecular Enzymology Group University of Groningen  Nijenborgh 4 Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-1002-0"},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-8083-6"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CS00903A"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb14983.x"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.112.1.527-531.1972"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(85)80004-7"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2016.100"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2012.09.003"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.11.5750-5755.2002"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09534-x"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b01506"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-8038-y"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-35590-2"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00070-15"},{"key":"e_1_2_2_15_1","first-page":"9","author":"Shah M. 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