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In particular, we have prepared several [1\u2010<jats:sup>13<\/jats:sup>C,<jats:sup>17<\/jats:sup>O]\u2010labeled yeast ubiquitin (Ub) samples including Ub\u2010[1\u2010<jats:sup>13<\/jats:sup>C,<jats:sup>17<\/jats:sup>O]Gly, Ub\u2010[1\u2010<jats:sup>13<\/jats:sup>C,<jats:sup>17<\/jats:sup>O]Tyr, and Ub\u2010[1\u2010<jats:sup>13<\/jats:sup>C,<jats:sup>17<\/jats:sup>O]Phe using the auxotrophic <jats:italic>E. coli<\/jats:italic> strain DL39 GlyA \u03bbDE3 (<jats:italic>asp<\/jats:italic>C<jats:sup>\u2212<\/jats:sup> <jats:italic>tyr<\/jats:italic>B<jats:sup>\u2212<\/jats:sup> <jats:italic>ilv<\/jats:italic>E<jats:sup>\u2212<\/jats:sup> <jats:italic>gly<\/jats:italic>A<jats:sup>\u2212<\/jats:sup> \u03bbDE3). We have also produced Ub\u2010[\u03b7\u2010<jats:sup>17<\/jats:sup>O]Tyr, in which the phenolic group of Tyr59 is <jats:sup>17<\/jats:sup>O\u2010labeled. We show for the first time that <jats:sup>17<\/jats:sup>O NMR signals from protein terminal residues and side chains can be readily detected in aqueous solution. We also reported solid\u2010state <jats:sup>17<\/jats:sup>O NMR spectra for Ub\u2010[1\u2010<jats:sup>13<\/jats:sup>C,<jats:sup>17<\/jats:sup>O]Tyr and Ub\u2010[1\u2010<jats:sup>13<\/jats:sup>C,<jats:sup>17<\/jats:sup>O]Phe obtained at an ultrahigh magnetic field, 35.2 T (1.5\u2005GHz for <jats:sup>1<\/jats:sup>H). This work represents a significant advance in the field of <jats:sup>17<\/jats:sup>O NMR studies of proteins.<\/jats:p>","DOI":"10.1002\/cbic.202000659","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T14:47:19Z","timestamp":1602773239000},"page":"826-829","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["<sup>17<\/sup>O NMR Studies of Yeast Ubiquitin in Aqueous Solution and in the Solid State"],"prefix":"10.1002","volume":"22","author":[{"given":"Binyang","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Chemistry Queen's University  Kingston ON K7L 3N6 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Hung","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Magnetic Resonance National High Magnetic Field Laboratory  Tallahassee FL 32310 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhehong","family":"Gan","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Magnetic Resonance National High Magnetic Field Laboratory  Tallahassee FL 32310 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po\u2010Hsiu","family":"Chien","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Magnetic Resonance National High Magnetic Field Laboratory  Tallahassee FL 32310 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly L.","family":"Spencer","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences Queen's University  Kingston ON K7L 3N6 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven P.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences Queen's University  Kingston ON K7L 3N6 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0936-9432","authenticated-orcid":false,"given":"Gang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Queen's University  Kingston ON K7L 3N6 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,6]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"crossref","unstructured":"K. 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