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The multi\u2010copper oxidase\/laccase CueO of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    is responsible for the oxidation of Cu\n                    <jats:sup>+<\/jats:sup>\n                    to the less harmful Cu\n                    <jats:sup>2+<\/jats:sup>\n                    in the periplasm. CueO has a relatively broad substrate spectrum as laccase, and its activity is enhanced by copper excess. The aim of this study was to trigger CueO activity\n                    <jats:italic>in\u2005vivo<\/jats:italic>\n                    for the use in biocatalysis. The addition of 5\u2005mM CuSO\n                    <jats:sub>4<\/jats:sub>\n                    was proven effective in triggering CueO activity at need with minor toxic effects on\n                    <jats:italic>E. coli<\/jats:italic>\n                    cells. Cu\u2010treated\n                    <jats:italic>E. coli<\/jats:italic>\n                    cells were able to convert several phenolic compounds to the corresponding dimers. Finally, the endogenous CueO activity was applied to a four\u2010step cascade, in which coniferyl alcohol was converted to the valuable plant lignan (\u2212)\u2010matairesinol.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202000775","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T06:28:42Z","timestamp":1608272922000},"page":"1470-1479","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Use of Copper as a Trigger for the\n                    <i>in Vivo<\/i>\n                    Activity of\n                    <i>E. coli<\/i>\n                    Laccase CueO: A Simple Tool for Biosynthetic Purposes"],"prefix":"10.1002","volume":"22","author":[{"given":"Davide","family":"Decembrino","sequence":"first","affiliation":[{"name":"Institute of Biochemistry Heinrich-Heine University D\u00fcsseldorf  Universit\u00e4tsstrasse 1 40225 D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Girhard","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry Heinrich-Heine University D\u00fcsseldorf  Universit\u00e4tsstrasse 1 40225 D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1312-4574","authenticated-orcid":false,"given":"Vlada B.","family":"Urlacher","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry Heinrich-Heine University D\u00fcsseldorf  Universit\u00e4tsstrasse 1 40225 D\u00fcsseldorf 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