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Retinitis pigmentosa (RP) is an inherited condition that is characterized by photoreceptor degeneration. Herein, we developed a fluorescent probe\u2010NeuA\u2010for detecting retinal neuronal cells and applied NeuA to discriminate between healthy and RP retinas. The staining pattern of NeuA in the retinas of healthy and RP mouse models was examined <jats:italic>in\u2005vitro<\/jats:italic>, <jats:italic>ex vivo<\/jats:italic> and <jats:italic>in\u2005vivo<\/jats:italic> using confocal microscopy, the fluorescent fundus microscopy and optical coherent tomography (OCT). NeuA strongly stained the outer segment layer of photoreceptor cells and some bipolar cells in the healthy retina, but there was only weak staining in the photoreceptor degenerated retinas. Therefore, NeuA probe can be used as the detecting RP tools in the preclinical conditions.<\/jats:p>","DOI":"10.1002\/cbic.202100011","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T08:36:52Z","timestamp":1613983012000},"page":"1915-1919","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Application of Neuron\u2010Selective Fluorescent Probe, NeuA, To Identify Mouse Retinal Degeneration"],"prefix":"10.1002","volume":"22","author":[{"given":"Sung\u2010Jin","family":"Park","sequence":"first","affiliation":[{"name":"Singapore Bioimaging Consortium Agency for Science Technology and Research  Singapore 138667 Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Queenie Tan Shu","family":"Woon","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology Agency for Science Technology and Research  Singapore 169856 Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Cheng","family":"Er","sequence":"additional","affiliation":[{"name":"Singapore Bioimaging Consortium Agency for Science Technology and Research  Singapore 138667 Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernice H.","family":"Wong","sequence":"additional","affiliation":[{"name":"Program in Cardiovascular and Metabolic Disorders Duke-NUS Medical School  Singapore 169857 Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Pohang University of Science and Technology (POSTECH)  Pohang 37673 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam\u2010Young","family":"Kang","sequence":"additional","affiliation":[{"name":"Singapore Bioimaging Consortium Agency for Science Technology and Research  Singapore 138667 Republic of Singapore"},{"name":"Department of Creative IT Engineering Pohang University of Science and Technology (POSTECH)  Pohang 37673 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veluchamy A.","family":"Barathi","sequence":"additional","affiliation":[{"name":"Singapore Eye Research Institute  Singapore 169856 Republic of Singapore"},{"name":"Eye-ACP, Duke-NUS Graduate Medical School  Singapore 169857 Republic of Singapore"},{"name":"Department of Ophthalmology, Yong Loo Lin School of Medicine National University of Singapore  Singapore 119228 Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Silver","sequence":"additional","affiliation":[{"name":"Program in Cardiovascular and Metabolic Disorders Duke-NUS Medical School  Singapore 169857 Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1927-3688","authenticated-orcid":false,"given":"Young\u2010Tae","family":"Chang","sequence":"additional","affiliation":[{"name":"Singapore Bioimaging Consortium Agency for Science Technology and Research  Singapore 138667 Republic of Singapore"},{"name":"Department of Chemistry Pohang University of Science and Technology (POSTECH)  Pohang 37673 Republic of Korea"},{"name":"Center for Self-assembly and Complexity Institute for Basic Science (IBS)  Pohang 37673 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,25]]},"reference":[{"key":"e_1_2_2_1_1","first-page":"01","volume-title":"The Organization of the Retina and Visaual System","author":"Kolb H.","year":"2005"},{"key":"e_1_2_2_2_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S1350-9462(97)00012-8","volume":"17","author":"Milam A. 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