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Here, we synthesized a cyclic pyrrole\u2010imidazole polyamide (cPIP), which can bind to the minor groove of the CAG\/CTG DNA sequence. The double\u2010stranded DNA melting temperature (<jats:italic>T<\/jats:italic><jats:sub>m<\/jats:sub>) and surface plasmon resonance assays revealed the high binding affinity of the cPIP. In addition, next\u2010generation sequencing showed that the cPIP had high specificity for its target DNA sequence.<\/jats:p>","DOI":"10.1002\/cbic.202100533","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T02:04:25Z","timestamp":1637287465000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Strong and Specific Recognition of CAG\/CTG Repeat DNA (5\u2019\u2010dWGCWGCW\u20103\u2019) by a Cyclic Pyrrole\u2010Imidazole Polyamide"],"prefix":"10.1002","volume":"23","author":[{"given":"Yuki","family":"Hirose","sequence":"first","affiliation":[{"name":"Department of Chemistry Graduate School of Science Kyoto University  Kitashirakawa-oiwakecho Sakyo-ku Kyoto 606-8502 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomo","family":"Ohno","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science Kyoto University  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