{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T08:50:22Z","timestamp":1768726222935,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T00:00:00Z","timestamp":1643241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2022,3,18]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we developed a new bienzymatic reaction to produce enantioenriched phenylethanols. In a first step, the recombinant, unspecific peroxygenase from <jats:italic>Agrocybe aegerita<\/jats:italic> (r<jats:italic>Aae<\/jats:italic>UPO) was used to oxidise ethylbenzene and its derivatives to the corresponding ketones (prochiral intermediates) followed by enantioselective reduction into the desired (<jats:italic>R<\/jats:italic>)\u2010 or (<jats:italic>S<\/jats:italic>)\u2010phenylethanols using the (<jats:italic>R<\/jats:italic>)\u2010selective alcohol dehydrogenase (ADH) from <jats:italic>Lactobacillus kefir<\/jats:italic> (<jats:italic>Lk<\/jats:italic>ADH) or the (<jats:italic>S<\/jats:italic>)\u2010selective ADH from <jats:italic>Rhodococcus ruber<\/jats:italic> (ADH\u2010A). In a one\u2010pot two\u2010step cascade, 11 ethylbenzene derivatives were converted into the corresponding chiral alcohols at acceptable yields and often excellent enantioselectivity.<\/jats:p>","DOI":"10.1002\/cbic.202200017","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T04:20:35Z","timestamp":1642047635000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Peroxygenase\u2010Alcohol Dehydrogenase Cascade Reaction to Transform Ethylbenzene Derivatives into Enantioenriched Phenylethanols"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5409-0211","authenticated-orcid":false,"given":"Xiaomin","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Biotechnology Delft University of Technology  2629HZ Delft (The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4506-5556","authenticated-orcid":false,"given":"Hugo","family":"Brasselet","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Delft University of Technology  2629HZ Delft (The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5256-4479","authenticated-orcid":false,"given":"Ewald P. J.","family":"Jongkind","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Delft University of Technology  2629HZ Delft (The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6780-7616","authenticated-orcid":false,"given":"Miguel","family":"Alcalde","sequence":"additional","affiliation":[{"name":"Department of Biocatalysis Institute of Catalysis, CSIC  28049 Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7889-9920","authenticated-orcid":false,"given":"Caroline E.","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Delft University of Technology  2629HZ Delft (The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4821-756X","authenticated-orcid":false,"given":"Frank","family":"Hollmann","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Delft University of Technology  2629HZ Delft (The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,27]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1039\/B703488C"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ed1003806"},{"key":"e_1_2_8_3_1","unstructured":"\u00a0"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-8083-6"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202001876"},{"key":"e_1_2_8_5_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202001876"},{"key":"e_1_2_8_6_1","unstructured":"\u00a0"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/cs500988a"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr020029g"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja983819"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja805128x"},{"key":"e_1_2_8_11_1","unstructured":"\u00a0"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2019.01.001"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2020.107615"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201510028"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201510028"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202100164"},{"key":"e_1_2_8_16_1","unstructured":"\u00a0"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.8.4575-4581.2004"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.oprd.1c00116"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2015.03.004"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00490-14"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo00031a037"},{"key":"e_1_2_8_22_1","unstructured":"\u00a0"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201300409"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol800549f"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo026216w"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6GC02157C"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2019.01.065"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcat.2021.112035"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0gc00918k"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2009.12.003"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2gc36797a"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/b98911"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202200017","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbic.202200017","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202200017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T11:32:11Z","timestamp":1760268731000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.202200017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,27]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,3,18]]}},"alternative-id":["10.1002\/cbic.202200017"],"URL":"https:\/\/doi.org\/10.1002\/cbic.202200017","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,27]]},"assertion":[{"value":"2022-01-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202200017"}}