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However to date, oxidation of secondary alcohols using alcohol oxidases has focused on activated benzylic or allylic substrates, with unactivated secondary alcohols showing poor activity. Here we show that cholesterol oxidase (EC\u20051.1.3.6) could be engineered for activity towards a range of aliphatic, cyclic, acyclic, allylic and benzylic secondary alcohols. Additionally, since the variants demonstrated high (<jats:italic>S<\/jats:italic>)\u2010selectivity, deracemisation reactions were performed in the presence of ammonia borane to obtain enantiopure (<jats:italic>R<\/jats:italic>)\u2010alcohols.<\/jats:p>","DOI":"10.1002\/cbic.202200075","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T13:04:02Z","timestamp":1644498242000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["An Engineered Cholesterol Oxidase Catalyses Enantioselective Oxidation of Non\u2010steroidal Secondary Alcohols"],"prefix":"10.1002","volume":"23","author":[{"given":"Rachel S.","family":"Heath","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Manchester Manchester Institute of Biotechnology  131 Princess Street M1 7DN Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack 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