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The resulting multivalent architectures were assayed as inhibitors of lysosomal GCase, the defective enzyme in Gaucher disease. The multivalent compounds resulted in much more potent inhibitors than a parent monovalent reference compound, thus showing a good multivalent effect. Biological investigation of these compounds as pharmacological chaperones revealed that the trivalent derivative (\n                    <jats:bold>12<\/jats:bold>\n                    ) gives a 2\u2010fold recovery of the GCase activity on Gaucher patient fibroblasts bearing the L444P\/L444P mutations responsible for neuropathies. 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