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In order to screen potential M<jats:sup>Pro<\/jats:sup> inhibitors we developed a luminescent assay using a peptide based probe containing a cleavage site specific for M<jats:sup>Pro<\/jats:sup>. This assay was validated showing IC<jats:sub>50<\/jats:sub> values similar to those reported in the literature for known M<jats:sup>Pro<\/jats:sup> inhibitors and can be used to screen new inhibitors.<\/jats:p>","DOI":"10.1002\/cbic.202200190","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T21:59:58Z","timestamp":1654120798000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Luminescent Assay for the Screening of SARS\u2010CoV\u20102 M<sup>Pro<\/sup> Inhibitors"],"prefix":"10.1002","volume":"23","author":[{"given":"Daan","family":"Sondag","sequence":"first","affiliation":[{"name":"Institute for Molecules and Materials Radboud University  6525 AJ Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jona","family":"Merx","sequence":"additional","affiliation":[{"name":"Institute for Molecules and Materials Radboud University  6525 AJ Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emiel","family":"Rossing","sequence":"additional","affiliation":[{"name":"Institute for Molecules and Materials Radboud University  6525 AJ Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9141-8784","authenticated-orcid":false,"given":"Thomas J.","family":"Boltje","sequence":"additional","affiliation":[{"name":"Institute for Molecules and Materials Radboud University  6525 AJ Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9284-837X","authenticated-orcid":false,"given":"Dennis W. 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