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To enhance this activity, known as pseudo\u2010peroxidase activity, previous studies have focused on the modification of key amino acid residues of Mb or the heme cofactor. In this work, the Mb scaffold (apo\u2010Mb) was systematically reconstituted with a set of cofactors based on six metal ions and two ligands. These Mb variants were fully characterised by UV\u2010Vis spectroscopy, circular dichroism (CD) spectroscopy, inductively coupled plasma mass spectrometry (ICP\u2010MS) and native mass spectrometry (nMS). The steady\u2010state kinetics of guaiacol oxidation and 2,4,6\u2010trichlorophenol (TCP) dehalogenation catalysed by Mb variants were determined. Mb variants with iron chlorin e6 (Fe\u2212Ce6) and manganese chlorin e6 (Mn\u2212Ce6) cofactors were found to have improved catalytic efficiency for both guaiacol and TCP substrates in comparison with wild\u2010type Mb, i.\u2009e. Fe\u2010protoporphyrin IX\u2010Mb. 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