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To maintain the polyketide biosynthetic fidelity, the KS domain must only accept an acyl group from the acyl carrier protein (ACP) domain of the immediate upstream module even when they are separated into different polypeptides. Although it was reported that both the docking domain\u2010based interactions and KS\u2010ACP compatibility are important for the interpolypeptide transacylation reaction in 6\u2010deoxyerythronolide B synthase, it is not clear whether these findings are broadly applied to other modular PKSs. Herein, we describe the importance of protein\u2010protein recognition in the intermodular transacylation between VinP1 module 3 and VinP2 module 4 in vicenistatin biosynthesis. We compared the transacylation activity and crosslinking efficiency of VinP2 KS<jats:sub>4<\/jats:sub> against the cognate VinP1 ACP<jats:sub>3<\/jats:sub> with the noncognate one. As a result, it appeared that VinP2 KS<jats:sub>4<\/jats:sub> distinguishes the cognate ACP<jats:sub>3<\/jats:sub> from other ACPs.<\/jats:p>","DOI":"10.1002\/cbic.202200200","type":"journal-article","created":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T22:09:58Z","timestamp":1651529398000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Protein\u2010Protein Recognition Involved in the Intermodular Transacylation Reaction in Modular Polyketide Synthase in the Biosynthesis of Vicenistatin"],"prefix":"10.1002","volume":"23","author":[{"given":"Taichi","family":"Chisuga","sequence":"first","affiliation":[{"name":"Department of Chemistry Tokyo Institute of Technology  2-12-1 O-okayama Meguro-ku Tokyo 152-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2219-6051","authenticated-orcid":false,"given":"Akimasa","family":"Miyanaga","sequence":"additional","affiliation":[{"name":"Department of Chemistry Tokyo Institute of Technology  2-12-1 O-okayama Meguro-ku Tokyo 152-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7830-7104","authenticated-orcid":false,"given":"Tadashi","family":"Eguchi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Tokyo Institute of Technology  2-12-1 O-okayama Meguro-ku Tokyo 152-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,16]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094318"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2np20019h"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jimb\/kuab018"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5413.482"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi002463n"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(03)00156-X"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","unstructured":"T.\u2005J. 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