{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:27:24Z","timestamp":1760315244681,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/M008770\/1"],"award-info":[{"award-number":["BB\/M008770\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["200021_192274"],"award-info":[{"award-number":["200021_192274"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2022,8,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enzymatic enantiopreference is one of the key advantages of biocatalysis. While exploring the synthesis of small cyclic (chiral amines) such as 3\u2010aminotetrahydrofuran (THF\u2010amine), using the (<jats:italic>S<\/jats:italic>)\u2010selective transaminase from <jats:italic>Halomonas elongata<\/jats:italic> (HEwT), inversion of the enantiopreference was observed at increasing substrate loadings. In addition, the enantiopreference could be altered by variation of the ionic strength, or of the co\u2010solvent content in the reaction mixture. For example, using otherwise identical reaction conditions, the presence of 2\u2005M sodium chloride gave (<jats:italic>R<\/jats:italic>)\u2010THF\u2010amine (14\u2009% <jats:italic>ee<\/jats:italic>), while the addition of 2.2\u2005M isopropyl alcohol gave the (<jats:italic>S<\/jats:italic>)\u2010enantiomer in 30\u2009% <jats:italic>ee<\/jats:italic>. While the underlying cause is not currently understood, it appears likely that subtle changes in the structure of the enzyme cause the shift in enantiopreference and are worth exploring further.<\/jats:p>","DOI":"10.1002\/cbic.202200335","type":"journal-article","created":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T22:10:01Z","timestamp":1655331001000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Influence of Reaction Conditions on Enzymatic Enantiopreference: the Curious Case of HEwT in the Synthesis of THF\u2010Amine"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0107-4477","authenticated-orcid":false,"given":"Christian M.","family":"Heckmann","sequence":"first","affiliation":[{"name":"School of Chemistry University of Nottingham University Park  Nottingham NG7 2RD 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