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Here, we report the formation of micro\u2010sized hexagons <jats:italic>via<\/jats:italic> assembly of only one pair of short double\u2010stranded DNA in buffer\u2010salt poly(ethylene glycol) solution. Each DNA strand had complementary bases with a two\u2010base overhang. The procedure of heating and subsequent cooling of blunt\u2010ended double\u2010stranded DNA resulted in different assemblies. These results indicated that end\u2010to\u2010end adhesion at the terminals induced by complementary overhangs were required to construct the hexagonal DNA assemblies. The stable formation of the hexagons was highly dependent on heating temperature. In addition, concentration adjustments of DNA and poly(ethylene glycol) were essential. Circular dichroism spectral measurements and polarization microscopy observations indicated parallel alignment of double\u2010stranded DNA in the hexagonal platelet. Self\u2010assembled micro\u2010sized hexagons composed of simple building blocks may have great potential for future biomedical device development.<\/jats:p>","DOI":"10.1002\/cbic.202200360","type":"journal-article","created":{"date-parts":[[2022,10,6]],"date-time":"2022-10-06T04:27:14Z","timestamp":1665030434000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Self\u2010Assembled Micro\u2010Sized Hexagons Built from Short DNA in a Crowded Environment"],"prefix":"10.1002","volume":"23","author":[{"given":"Tetsunao","family":"Makino","sequence":"first","affiliation":[{"name":"Department of Engineering Science Graduate School of Informatics and Engineering The University of Electro-Communications 1-5-1 Chofugaoka, Chofu  Tokyo 182-8585 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8201-2608","authenticated-orcid":false,"given":"Daisuke","family":"Nakane","sequence":"additional","affiliation":[{"name":"Department of Engineering Science Graduate School of Informatics and Engineering The University of Electro-Communications 1-5-1 Chofugaoka, Chofu  Tokyo 182-8585 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5819-802X","authenticated-orcid":false,"given":"Makiko","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Engineering Science Graduate School of Informatics and Engineering The University of Electro-Communications 1-5-1 Chofugaoka, Chofu  Tokyo 182-8585 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0205353"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/sca.4950240505"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/356776a0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crci.2007.09.008"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(97)78207-9"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/339724a0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10867-016-9433-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.8.1886"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1088\/0953-8984\/20\/03\/035102"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cplett.2018.07.028"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4371(91)90436-G"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0901240106"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7SM00384F"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10769"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03745-4"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat4909"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201500610"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201701019"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201809757"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201809757"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202005505"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202005505"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CC07458F"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c02456"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1117463109"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1088\/0953-8984\/20\/49\/494214"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1OB01669E"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053305"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6700(96)00016-0"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9CC02255D"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/02678299208029034"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04586"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2NR32393A"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c06963"},{"key":"e_1_2_8_33_1","first-page":"18","author":"Glaser M.","year":"2016","journal-title":"New J. 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