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In this study, a simple and versatile method for chemoenzymatic labeling of intercellularly contacting cells is demonstrated using a cell\u2010surface anchoring reagent of a poly(ethylene glycol)(PEG)\u2010lipid conjugate. The surface of each cell in the cell pairs of interest were decorated with sortase A (SrtA) and triglycine peptide that were lipidated with PEG\u2010lipid. In the mixture of the two\u2010cell populations, the triglycine\u2010modified cells were enzymatically labeled with a fluorescent labeling reagent when in contact with SrtA\u2010modified cells on a substrate. The selective labeling of the contacting cells was confirmed by confocal microscopy. The method is a promising tool for selective visualization of intercellularly contacting cells in cell mixtures for cell\u2010cell communication analysis.<\/jats:p>","DOI":"10.1002\/cbic.202200474","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T13:19:32Z","timestamp":1660742372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Chemoenzymatic Labeling to Visualize Intercellular Contacts Using Lipidated SortaseA**"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4822-7469","authenticated-orcid":false,"given":"Satoshi","family":"Yamaguchi","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biotechnology The University of Tokyo  7-3-1 Hongo, Bunkyo-ku Tokyo 113-8656 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryosuke","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of 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