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This bioresponsive probe exhibits pH\u2010sensitive reversible absorption\/emission features, low cytotoxicity, a huge 322\u2005nm bathochromic spectral shift with augmented quantum yield from neutral to acidic pH, high sensitivity and selective targeting ability of live\u2010cell lysosomes with ideal p<jats:italic>K<\/jats:italic><jats:sub>a<\/jats:sub>, off\u2010to\u2010on narrow NIR absorption\/fluorescence signals with high molar absorption coefficient at acidic lysosomal lumen, and in\u2010situ live\u2010cell pH\u2010activated ratiometric imaging of lysosomal pH. Selective staining and ratiometric pH imaging in human carcinoma live\u2010cell lysosomes were monitored by dual\u2010channel confocal laser scanning microscope using a pH\u2010activatable organic fluorescent dye comprising a morpholine moiety for lysosome targeting and an acidic pH openable oxazolidine ring. Moreover, real\u2010time tracking of lysosomes, 3D, and multicolor live\u2010cell imaging have been achieved using the synthesized pH\u2010activatable probe.<\/jats:p>","DOI":"10.1002\/cbic.202200641","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T11:29:40Z","timestamp":1669980580000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Acidic pH\u2010Triggered Live\u2010Cell Lysosome Specific Tracking, Ratiometric pH Sensing, and Multicolor Imaging by Visible to NIR Switchable Cy\u20107 Dyes"],"prefix":"10.1002","volume":"24","author":[{"given":"Ayan","family":"Mukherjee","sequence":"first","affiliation":[{"name":"Department of Chemistry Organic Chemistry Section Jadavpur University  Kolkata 700032 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pranab Chandra","family":"Saha","sequence":"additional","affiliation":[{"name":"Department of Chemistry Organic 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