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In this study, we identified the virustomycin\u2005A biosynthetic gene cluster, which encodes type\u2005I polyketide synthases (PKSs), ethylmalonyl\u2010CoA biosynthetic enzymes, methoxymalony\u2010acyl carrier protein biosynthetic enzymes, and post\u2010PKS modification enzymes. Next, we demonstrated that the acyltransferase domain can be exchanged between the Vsm PKSs and the PKSs involved in FD\u2010891 biosynthesis (Gfs PKSs), without any supply problems of the unique extender units. We exchanged the malonyltransferase domain in the loading module of Gfs PKS with the ethylmalonyltransferase domain and the methoxymalonyltransferase domain of Vsm PKSs. Consequently, the expected two\u2010carbon\u2010elongated analog 26\u2010ethyl\u2010FD\u2010891 was successfully produced with a titer comparable to FD\u2010891 production by the wild type; however, exchange with the methoxymalonyltransferase domain did not produce any FD\u2010891 analogs. Furthermore, 26\u2010ethyl\u2010FD\u2010891 showed potent cytotoxic activity against HeLa cells, like natural FD\u2010891.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202200670","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T06:35:53Z","timestamp":1672900553000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Acyltransferase Domain Exchange between Two Independent Type\u2005I Polyketide Synthases in the Same Producer Strain of Macrolide Antibiotics"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4788-0063","authenticated-orcid":false,"given":"Fumitaka","family":"Kudo","sequence":"first","affiliation":[{"name":"Department of Chemistry Tokyo Institute of Technology  2-12-1 Meguro-ku, O-okayama Tokyo 152-8551 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