{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:03:01Z","timestamp":1780401781139,"version":"3.54.1"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:00:00Z","timestamp":1682553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BO 1862\/23-1"],"award-info":[{"award-number":["BO 1862\/23-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2023,5,16]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Baeyer\u2010Villiger monooxygenases (BVMOs) are important flavin\u2010dependent enzymes which perform oxygen insertion reactions leading to valuable products. As reported in many studies, BVMOs are usually unstable during application, preventing a wider usage in biocatalysis. Here, we discovered a novel NADPH\u2010dependent BVMO which originates from <jats:italic>Halopolyspora algeriensis<\/jats:italic> using sequence similarity networks (SSNs). The enzyme is stable at temperatures between 10\u2009\u00b0C to 30\u2009\u00b0C up to five days after the purification, and yields the normal ester product. In this study, the substrate scope was investigated for a broad range of aliphatic ketones and the enzyme was biochemically characterized to identify optimum reaction conditions. The best substrate (86\u2009% conversion) was 2\u2010dodecanone using purified enzyme. This novel BVMO could potentially be applied as part of an enzymatic cascade or in bioprocesses which utilize aliphatic alkanes as feedstock.<\/jats:p>","DOI":"10.1002\/cbic.202200746","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T04:28:58Z","timestamp":1678854538000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Discovery and Characterization of a Baeyer\u2010Villiger Monooxygenase Using Sequence Similarity Network Analysis"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3138-9357","authenticated-orcid":false,"given":"Thaleia","family":"Sakoleva","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Enzyme Catalysis University of Greifswald  Felix-Hausdorff-Stra\u00dfe 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1874-6564","authenticated-orcid":false,"given":"Harry P.","family":"Austin","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Enzyme Catalysis University of Greifswald  Felix-Hausdorff-Stra\u00dfe 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chrysoula","family":"Tzima","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Enzyme Catalysis University of Greifswald  Felix-Hausdorff-Stra\u00dfe 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3270-6895","authenticated-orcid":false,"given":"Mark","family":"D\u00f6rr","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Enzyme Catalysis University of Greifswald  Felix-Hausdorff-Stra\u00dfe 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0685-2696","authenticated-orcid":false,"given":"Uwe T.","family":"Bornscheuer","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Enzyme Catalysis University of Greifswald  Felix-Hausdorff-Stra\u00dfe 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,27]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1007\/s00253-011-3670-z","volume":"94","author":"Leipold F.","year":"2012","journal-title":"Appl. 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