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With the development of psoralen\u2010conjugated oligonucleotides (Ps\u2010Oligos), sequence\u2010specific crosslinking with target DNA has become achievable, thereby expanding the application of psoralen\u2010conjugated molecules in gene transcription inhibition, gene knockout, and targeted recombination by genome editing. In this study, we developed two novel psoralen <jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide (NHS) esters that allow the introduction of psoralens into any amino\u2010modified oligonucleotides. Quantitative evaluation of the photo\u2010crosslinking efficiencies of the Ps\u2010Oligos to target single\u2010stranded DNAs revealed that the crosslinking selectivity to 5\u2010mC is the unique feature of trioxsalen. We found that the introduction of an oligonucleotide <jats:italic>via<\/jats:italic> a linker at the C\u20105 position of psoralen can promote favorable crosslinking to target double\u2010stranded DNA. We believe our findings are essential information for the development of Ps\u2010Oligos as novel gene regulation tools.<\/jats:p>","DOI":"10.1002\/cbic.202200789","type":"journal-article","created":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T04:02:43Z","timestamp":1678420963000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Unique Crosslinking Properties of Psoralen\u2010Conjugated Oligonucleotides Developed by Novel Psoralen <i>N<\/i>\u2010Hydroxysuccinimide Esters"],"prefix":"10.1002","volume":"24","author":[{"given":"Juki","family":"Nakao","sequence":"first","affiliation":[{"name":"Graduate School of Biomedical Sciences Nagasaki University  1-14 Bunkyo-machi, Nagasaki Nagasaki 852-8521 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Mikame","sequence":"additional","affiliation":[{"name":"Graduate School of Biomedical Sciences Nagasaki University  1-14 Bunkyo-machi, 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