{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:47:41Z","timestamp":1790743661457,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The type 2 secretion system (T2SS) is a bacterial nanomachine composed of an inner membrane assembly platform, an outer membrane pore and a dynamic endopilus. T2SS endopili are organized into a homo\u2010multimeric body formed by the major pilin capped by a heterocomplex of four minor pilins. The first model of the T2SS endopilus was recently released, even if structural dynamics insights are still required to decipher the role of each protein in the full tetrameric complex. Here, we applied continuous\u2010wave and pulse EPR spectroscopy using nitroxide\u2010gadolinium orthogonal labelling strategies to investigate the hetero\u2010oligomeric assembly of the minor pilins. Overall, our data are in line with the endopilus model even if they evidenced conformational flexibility and alternative orientations at local scale of specific regions of minor pilins. The integration of different labelling strategies and EPR experiments demonstrates the pertinence of this approach to investigate protein\u2013protein interactions in such multiprotein heterocomplexes.<\/jats:p>","DOI":"10.1002\/cbic.202300099","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T04:59:03Z","timestamp":1680238743000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Refining the Dynamic Network of T2SS Endopilus Tip Heterocomplex Combining cw\u2010EPR and Nitroxide\u2013Gd<sup>III<\/sup> Distance Measurements"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7320-9839","authenticated-orcid":false,"given":"Guillaume","family":"Gerbaud","sequence":"first","affiliation":[{"name":"BIP-Bio\u00e9n\u00e9rgetique et Ing\u00e9nierie es Prot\u00e9ines IMM Aix Marseille Universit\u00e9  CNRS 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brice","family":"Barbat","sequence":"additional","affiliation":[{"name":"LCB-Laboratoire de Chimie Bact\u00e9rienne IMM Aix Marseille Universit\u00e9  CNRS 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilde","family":"Tribout","sequence":"additional","affiliation":[{"name":"LCB-Laboratoire de Chimie Bact\u00e9rienne IMM Aix Marseille Universit\u00e9  CNRS 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilien","family":"Etienne","sequence":"additional","affiliation":[{"name":"BIP-Bio\u00e9n\u00e9rgetique et Ing\u00e9nierie es Prot\u00e9ines IMM Aix Marseille Universit\u00e9  CNRS 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Belle","sequence":"additional","affiliation":[{"name":"BIP-Bio\u00e9n\u00e9rgetique et Ing\u00e9nierie es Prot\u00e9ines IMM Aix Marseille Universit\u00e9  CNRS 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3140-4713","authenticated-orcid":false,"given":"Badreddine","family":"Douzi","sequence":"additional","affiliation":[{"name":"LCB-Laboratoire de Chimie Bact\u00e9rienne IMM Aix Marseille Universit\u00e9  CNRS 13009 Marseille France"},{"name":"Present address: INRAE, DynAMic Universit\u00e9 de Lorraine  54000 Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5258-5958","authenticated-orcid":false,"given":"Rom\u00e9","family":"Voulhoux","sequence":"additional","affiliation":[{"name":"LCB-Laboratoire de Chimie Bact\u00e9rienne IMM Aix Marseille Universit\u00e9  CNRS 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4226-2864","authenticated-orcid":false,"given":"Alessio","family":"Bonucci","sequence":"additional","affiliation":[{"name":"BIP-Bio\u00e9n\u00e9rgetique et Ing\u00e9nierie es Prot\u00e9ines IMM Aix Marseille Universit\u00e9  CNRS 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,28]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.4.1059-1071.1999"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20020194"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1426"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.042366"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuu001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007343"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2021.05.015"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10858-019-00246-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2021.140653"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2020.108323"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2013-0155"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1039\/9781788013888-00066","volume-title":"Electron Paramagnetic Resonance, Vol. 26","author":"Martinho M.","year":"2018"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201102539"},{"key":"e_1_2_9_14_1","volume":"563","author":"Schmidt M. 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