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FasT possesses an unusual off\u2010loading domain, which was heterologously expressed in <jats:italic>E. coli<\/jats:italic> and found to act as an \u03b1\u2010oxoamine synthase (AOS) <jats:italic>in\u2005vitro<\/jats:italic>. Similar to serine palmitoyltransferases from sphingolipid biosynthesis, the AOS off\u2010loading domain catalyzes a decarboxylative Claisen condensation between <jats:sc>l<\/jats:sc>\u2010serine and a fatty acyl thioester. While the AOS domain was strictly specific for <jats:sc>l<\/jats:sc>\u2010serine, thioesters with saturated fatty acyl chains of six carbon atoms and longer were tolerated, with the highest activity observed for stearoyl\u2212coenzyme A (C<jats:sub>18<\/jats:sub>). Our findings suggest a novel route to \u03b1\u2010amino ketones <jats:italic>via<\/jats:italic> the direct condensation of iteratively produced long\u2010chain fatty acids with <jats:sc>l<\/jats:sc>\u2010serine by a FAS with a <jats:italic>cis<\/jats:italic>\u2010acting AOS off\u2010loading domain.<\/jats:p>","DOI":"10.1002\/cbic.202300209","type":"journal-article","created":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T03:40:33Z","timestamp":1683258033000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of an Unusual \u03b1\u2010Oxoamine Synthase Off\u2010Loading Domain from a Cyanobacterial Type I Fatty Acid Synthase"],"prefix":"10.1002","volume":"24","author":[{"given":"Andrei","family":"Ogonkov","sequence":"first","affiliation":[{"name":"Department of Biology, Institute of Microbiology ETH Zurich  Vladimir-Prelog-Weg 4 8093 Zurich Switzerland"},{"name":"Institute of Biology Leipzig 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