{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T19:39:38Z","timestamp":1787600378330,"version":"build-2736575974"},"reference-count":15,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T00:00:00Z","timestamp":1691712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009377","name":"Studium Loire Valley-Institute for Advanced Studies","doi-asserted-by":"publisher","award":["MIACYC-ANR-20-CE43-0010"],"award-info":[{"award-number":["MIACYC-ANR-20-CE43-0010"]}],"id":[{"id":"10.13039\/501100009377","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2023,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cocaine and hyoscyamine are two tropane alkaloids (TA) from Erythroxylaceae and Solanaceae, respectively. These famous compounds possess anticholinergic properties that can be used to treat neuromuscular disorders. While the hyoscyamine biosynthetic pathway has been fully elucidated allowing its de novo synthesis in yeast, the cocaine pathway remained only partially elucidated. Recently, the Huang research group has completed the cocaine biosynthetic route by characterizing its two missing enzymes. This allowed the whole pathway to be transferring into <jats:italic>Nicotiana benthamiana<\/jats:italic> to achieve cocaine production. Here, besides highlighting the impact of this discovery, we discuss how TA biosynthesis evolved via the recruitment of two distinct and convergent pathways in Erythroxylaceae and Solanaceae. Finally, while enriching our knowledge on TA biosynthesis, this diversification of the molecular actors involved in cocaine and hyoscyamine biosynthesis opens perspectives in metabolic engineering by exploring enzyme biochemical plasticity that can ease and shorten TA pathway reconstitution in heterologous organisms.<\/jats:p>","DOI":"10.1002\/cbic.202300234","type":"journal-article","created":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T06:33:53Z","timestamp":1685428433000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Evolutionary Pattern of Cocaine and Hyoscyamine Biosynthesis Provides Strategies To Produce Tropane Alkaloids"],"prefix":"10.1002","volume":"24","author":[{"given":"Duchesse\u2010Lacours","family":"Zamar","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Tours, Facult\u00e9 de Pharmacie EA2106 Biomol\u00e9cules et Biotechnologies V\u00e9g\u00e9tales  31, Avenue Monge 37200 Tours France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6265-7321","authenticated-orcid":false,"given":"Nicolas","family":"Papon","sequence":"additional","affiliation":[{"name":"Universit\u00e9 d'Angers Fungal Respiratory Infections Research Unit University Hospital of Angers  4 rue de Larrey 49933 Angers Cedex 09 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8902-4532","authenticated-orcid":false,"given":"Vincent","family":"Courdavault","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tours, Facult\u00e9 de Pharmacie EA2106 Biomol\u00e9cules et Biotechnologies V\u00e9g\u00e9tales  31, Avenue Monge 37200 Tours France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,11]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13659-022-00357-w"},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-012-9886-1"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32776-1"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200473109"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.114.248187"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/b001713m"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c09091"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aob.a086511"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.682181"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24040796"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2215372119"},{"key":"e_1_2_2_12_1","unstructured":"The Angiosperm Phylogeny Group Bot. 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