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Curli promote adhesion to abiotic surfaces and plant and human host tissues and are associated with pathogenesis in urinary tract infection and food\u2010borne illness. The production of curli in the host has also been implicated in the pathogenesis of neurodegenerative diseases. We report that the natural product nordihydroguaiaretic acid (NDGA) is effective as a curlicide in <jats:italic>E. coli<\/jats:italic>. NDGA prevents CsgA polymerization in\u2005vitro in a dose\u2010dependent manner. NDGA selectively inhibits cell\u2010associated curli assembly and inhibits uropathogenic <jats:italic>E. coli<\/jats:italic> biofilm formation. More broadly, this work emphasizes the ability to evaluate and identify bioactive amyloid assembly inhibitors by using the powerful gene\u2010directed amyloid biogenesis machinery in <jats:italic>E. coli<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/cbic.202300266","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T08:36:47Z","timestamp":1684312607000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Nordihydroguaiaretic Acid (NDGA) Inhibits CsgA Polymerization, Bacterial Amyloid Biogenesis, and Biofilm Formation"],"prefix":"10.1002","volume":"24","author":[{"given":"Joshua A.","family":"Visser","sequence":"first","affiliation":[{"name":"Department of Chemistry Stanford University  380 Roth Way, Keck Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Yager","sequence":"additional","affiliation":[{"name":"Department of 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