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Here, we extend the spectrum of suitable enzymes by three alcohol dehydrogenases from <jats:italic>Citrifermentans bemidjiense<\/jats:italic> (CibADH), <jats:italic>Deferrisoma camini<\/jats:italic> (DecADH), and <jats:italic>Thauera phenylacetica<\/jats:italic> (ThpADH). Of these, DecADH is characterized by very high thermostability; CibADH and ThpADH convert \u03b1\u2010halogenated cyclohexanones with increased activity. Otherwise, however, the substrate spectrum of all four ADHs is highly conserved. Structural considerations led to the conclusion that conversion of diketones requires not only the expansion of the active site into a large binding pocket, but also the circumferential modification of almost all amino acid residues that form the first shell of the binding pocket. The constellation appears to be overall highly specific for the relative positioning of the carbonyl functions and the size of the C\u2010ring.<\/jats:p>","DOI":"10.1002\/cbic.202300290","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T13:05:28Z","timestamp":1683810328000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Extended Scope and Understanding of Zinc\u2010Dependent Alcohol Dehydrogenases for Reduction of Cyclic \u03b1\u2010Diketones"],"prefix":"10.1002","volume":"24","author":[{"given":"Frances","family":"Stark","sequence":"first","affiliation":[{"name":"Professur f\u00fcr Molekulare Biotechnologie Technische Universit\u00e4t Dresden  01062 Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Professur f\u00fcr Molekulare Biotechnologie Technische Universit\u00e4t Dresden  01062 Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadine","family":"Ihle","sequence":"additional","affiliation":[{"name":"Professur f\u00fcr Molekulare Biotechnologie Technische Universit\u00e4t Dresden  01062 Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Loderer","sequence":"additional","affiliation":[{"name":"Professur f\u00fcr Molekulare Biotechnologie Technische Universit\u00e4t Dresden  01062 Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2912-546X","authenticated-orcid":false,"given":"Marion B.","family":"Ansorge\u2010Schumacher","sequence":"additional","affiliation":[{"name":"Professur f\u00fcr Molekulare Biotechnologie Technische Universit\u00e4t Dresden  01062 Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,27]]},"reference":[{"key":"e_1_2_9_1_1","unstructured":"\u00a0"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-1002-0"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-1005-x"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0957-4166(03)00526-3"},{"key":"e_1_2_9_5_1","unstructured":"\u00a0"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2008.06.019"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.104644"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10562-019-03015-y"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b00621"},{"key":"e_1_2_9_10_1","unstructured":"\u00a0"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo960932e"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.49.932"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(02)01058-2"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar980062i"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)74046-9"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar960222v"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0957-4166(98)00165-7"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(99)00548-X"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(99)00035-6"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2003.10.017"},{"key":"e_1_2_9_21_1","unstructured":"\u00a0"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7CY00169J"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00409663"},{"key":"e_1_2_9_24_2","unstructured":"T. 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