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In this work, we utilized linear and \u039b\u2010shaped polarity\u2010sensitive fluorescent probes for imaging LDs in both cellular and tissue environments, specifically in rats with diabetic and alcoholic fatty liver disease. The fluorescent probes possess several key characteristics, including high permeability, lipophilicity, and brightness, which make them well\u2010suited for efficient LD imaging. Notably, the probes exhibit a substantial Stokes shift, with 143\u2005nm for DCS and 201\u2005nm for DCN with selective targeting of the lipid droplets. Our experimental investigations successfully differentiated morphological variations between diseased and normal tissues in three distinct tissue types: liver, adipose, and small intestine. 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