{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:35:58Z","timestamp":1786977358133,"version":"build-2736575974"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SE\u2005609\/19-1"],"award-info":[{"award-number":["SE\u2005609\/19-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["NI\u2005694\/10-1"],"award-info":[{"award-number":["NI\u2005694\/10-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2024,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Flavin\u2010dependent halogenases allow halogenation of electron\u2010rich aromatic compounds under mild reaction conditions even at electronically unfavored positions with high regioselectivity. In order to expand the application of halogenases, the enzymes need to be improved in terms of stability and efficiency. A previous study with the tryptophan 6\u2010halogenase Thal demonstrated that thermostable Thal variants tend to form dimers in solution while the wild type is present as a monomer. Based on this a dimeric Thal variant was generated that is covalently linked by disulfide bonds. Introducing two cysteine residues at the dimer interface resulted in the variant Thal\u2005CC with significantly increased thermostability (\u25b5\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>50<\/jats:sub>\n                    =15.7\u2005K) and stability over time at elevated temperature compared to the wild type. By introducing the homologous mutations into the tryptophan 5\u2010halogenase PyrH, we were able to show that the stabilization by covalent dimerization can also be transferred to other halogenases. Moreover, it was possible to further increase the thermostability of PyrH by inserting cysteine mutations at alternative sites of the dimer interface.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202300700","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T11:44:15Z","timestamp":1698925455000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Increasing the Stability of Flavin\u2010Dependent Halogenases by Disulfide Engineering"],"prefix":"10.1002","volume":"25","author":[{"given":"Caroline","family":"Besse","sequence":"first","affiliation":[{"name":"Organic and Bioorganic Chemistry Department of Chemistry Bielefeld University  Universit\u00e4tsstra\u00dfe 25 33615 Bielefeld Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8582-0360","authenticated-orcid":false,"given":"Hartmut H.","family":"Niemann","sequence":"additional","affiliation":[{"name":"Structural Biochemistry Department of Chemistry Bielefeld University  Universit\u00e4tsstra\u00dfe 25 33615 Bielefeld Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0309-2655","authenticated-orcid":false,"given":"Norbert","family":"Sewald","sequence":"additional","affiliation":[{"name":"Organic and Bioorganic Chemistry Department of Chemistry Bielefeld University  Universit\u00e4tsstra\u00dfe 25 33615 Bielefeld Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,20]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.3390\/md13074044"},{"key":"e_1_2_9_2_1","volume-title":"Naturally occurring organohalogen compounds-a comprehensive update","author":"Gribble G. 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