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Here, we designed and developed photo\u2010click morphine (PCM\u20102) as a photo\u2010affinity probe based on morphine and dialkynyl\u2010acetyl morphine (DAAM) as a metabolic acetate reporter based on heroin. Application of these probes to SH\u2010SY5Y, HEK293T, and U2OS cells revealed that PCM\u20102 and DAAM primarily localize to the lysosome amongst other locations inside the cell by confocal microscopy and chemical proteomics. Interaction site identification by mass spectrometry revealed the mitochondrial phosphate carrier protein, solute carrier family 25 member 3, SLC25A3, and histone H2B as acylation targets of DAAM. 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