{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:35:57Z","timestamp":1786977357347,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T00:00:00Z","timestamp":1715644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2024,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The widespread attention towards 1,4\u2010butanediol (BDO) as a key chemical raw material stems from its potential in producing biodegradable plastics. However, the efficiency of its biosynthesis via current bioprocesses is limited. In this study, a dual\u2010pathway approach for 1,4\u2010BDO production from succinic acid was developed. Specifically, a double\u2010enzyme catalytic pathway involving carboxylic acid reductase and ethanol dehydrogenase was proposed. Optimization of the expression levels of the pathway enzymes led to a significant 318\u2009% increase in 1,4\u2010BDO titer. Additionally, the rate\u2010limiting enzyme\n                    <jats:italic>Mm<\/jats:italic>\n                    CAR was engineered to enhance the\n                    <jats:italic>\n                      k\n                      <jats:sub>cat<\/jats:sub>\n                      \/K\n                      <jats:sub>M<\/jats:sub>\n                    <\/jats:italic>\n                    values by 50\u2009% and increase 1,4\u2010BDO titer by 46.7\u2009%. To address cofactor supply limitations, an NADPH and ATP cycling system was established, resulting in a 48.9\u2009% increase in 1,4\u2010BDO production. Ultimately, after 48\u2005hours, 1,4\u2010BDO titers reached 201\u2005mg\/L and 1555\u2005mg\/L in shake flask and 5\u2005L fermenter, respectively. This work represents a significant advancement in 1,4\u2010BDO synthesis from succinic acid, with potential applications in the organic chemical and food industries.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202400142","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T09:46:33Z","timestamp":1715679993000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Production of 1,4\u2010Butanediol from Succinic Acid Using\n                    <i>Escherichia Coli<\/i>\n                    Whole\u2010Cell Catalysis"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8050-1288","authenticated-orcid":false,"given":"Ping","family":"Ni","sequence":"first","affiliation":[{"name":"School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education Jiangnan University  Wuxi 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214122 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqing","family":"Wei","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education Jiangnan University  Wuxi 214122"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiulai","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education Jiangnan University  Wuxi 214122"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3022-936X","authenticated-orcid":false,"given":"Liming","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education Jiangnan University  Wuxi 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