{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:07:06Z","timestamp":1789240026371,"version":"build-2803163510"},"reference-count":62,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T00:00:00Z","timestamp":1716249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22071072"],"award-info":[{"award-number":["22071072"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21801086"],"award-info":[{"award-number":["21801086"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2024,6,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chiral alcohols are not only important building blocks of various bioactive natural compounds and pharmaceuticals, but can serve as synthetic precursors for other valuable organic chemicals, thus the synthesis of these products is of great importance. Bio\u2010catalysis represents one effective way to obtain these molecules, however, the weak stability and high cost of enzymes often hinder its broad application. In this work, we designed a biological nanoreactor by embedding alcohol dehydrogenase (ADH) and glucose dehydrogenase (GDH) in metal\u2010organic\u2010framework ZIF\u20108. The biocatalyst ADH&amp;GDH@ZIF\u20108 could be applied to the asymmetric reduction of a series of ketones to give chiral alcohols in high yields (up to 99\u2009%) and with excellent enantioselectivities (&gt;99\u2009%). In addition, the heterogeneous biocatalyst could be recycled and reused at least four times with slight activity decline. Moreover,\n                    <jats:italic>E.\u2005coli<\/jats:italic>\n                    containing ADH and GDH was immobilized by ZIF\u20108 to form biocatalyst\n                    <jats:italic>E.\u2005coli<\/jats:italic>\n                    @ZIF\u20108, which also exhibits good catalytic behaviours. Finally, the chiral alcohols are further converted to marketed drugs (\n                    <jats:italic>R<\/jats:italic>\n                    )\u2010Fendiline, (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010Rivastigmine and NPS\n                    <jats:italic>R\u2010568<\/jats:italic>\n                    respectively.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202400147","type":"journal-article","created":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T04:34:39Z","timestamp":1713328479000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Co\u2010Immobilization of ADH and GDH on Metal\u2013Organic\u2010Framework: An Effective Biocatalyst for Asymmetric Reduction of Ketones"],"prefix":"10.1002","volume":"25","author":[{"given":"Lu","family":"Ran","sequence":"first","affiliation":[{"name":"College of Chemistry Huazhong Agricultural University  Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Chemistry Huazhong Agricultural University  Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guorong","family":"Su","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Huazhong Agricultural University  Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyan","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Chemistry Huazhong Agricultural University  Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7544-2856","authenticated-orcid":false,"given":"Huailong","family":"Teng","sequence":"additional","affiliation":[{"name":"College of Chemistry Huazhong Agricultural University  Wuhan 430070 P. R. 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