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A bioactivity\u2010guided discovery process led to the identification of the new melleolide 5\u2019\u2010methoxyarmillane (\n                    <jats:bold>1<\/jats:bold>\n                    ) in organic extracts from the mycelium of\n                    <jats:italic>Armillaria ostoyae<\/jats:italic>\n                    . Remarkably, supplementation of rapeseed oil to the culture medium potato dextrose broth increased the production of\n                    <jats:bold>1<\/jats:bold>\n                    by a factor of six during the course of the 35\u2005days fermentation. Compound\n                    <jats:bold>1<\/jats:bold>\n                    was isolated and its structure elucidated by UHPLC\u2010QTOF\u2010HR\u2010MS\/MS and NMR spectroscopy. It showed toxicity against Madin\u2010Darby canine kidney\u2005II (MDCK\u2005II, IC\n                    <jats:sub>50<\/jats:sub>\n                    19.2\u2005\u03bcg\/mL, 44.1\u2005\u03bcM) and human lung cancer Calu\u20103 cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    15.2\u2005\u03bcg\/mL, 34.9\u2005\u03bcM) as well as moderate bioactivity against\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    (MIC 8\u2005mg\/mL, 18.4\u2005\u03bcM) and\n                    <jats:italic>Mycobacterium smegmatis<\/jats:italic>\n                    (MIC 16\u2005mg\/mL, 36.8\u2005\u03bcM), but not against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , and\n                    <jats:italic>Septoria tritici<\/jats:italic>\n                    . No inhibitory effects of\n                    <jats:bold>1<\/jats:bold>\n                    against the influenza viruses H3N2, H1N1pdm, B\/Malaysia, and B\/Massachusetts were observed.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202400168","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T06:38:44Z","timestamp":1715582324000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["5\u2019\u2010Methoxyarmillane, a Bioactive Sesquiterpenoid Aryl Ester from the Fungus\n                    <i>Armillaria ostoyae<\/i>"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3371-6377","authenticated-orcid":false,"given":"Axel M.","family":"Orban","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Bioresources  35392 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Eichberg","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Bioresources  35392 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1024-1567","authenticated-orcid":false,"given":"Michael","family":"Marner","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Bioresources  35392 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Breuer","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Bioresources  35392 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A.","family":"Patras","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Bioresources  35392 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Mettal","sequence":"additional","affiliation":[{"name":"Institute for Insect Biotechnology Division for Natural Product Research Justus-Liebig-University Giessen  35392 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9947-8079","authenticated-orcid":false,"given":"Till F.","family":"Sch\u00e4berle","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Bioresources  35392 Giessen Germany"},{"name":"Institute for Insect Biotechnology Division for Natural Product Research Justus-Liebig-University Giessen  35392 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8274-8175","authenticated-orcid":false,"given":"Martin","family":"R\u00fchl","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Bioresources  35392 Giessen Germany"},{"name":"Institute of Food Chemistry and Food Biotechnology Justus-Liebig-University Giessen  35392 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,8]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4NP00075G"},{"key":"e_1_2_9_2_1","first-page":"1","author":"Chen H.-Y.","year":"2022","journal-title":"Crit. 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