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Oils from waste and enzymatic catalysis have proven to be good solutions to these problems. Lipases are currently the most commonly used enzymes in the transesterification of oils; nevertheless, enzymes have a high cost and must be immobilized to offer repetitive reuse. This work focuses on the synthesis and the optimization of the conditions of a xerogel where the lipase from <jats:italic>Candida antarctica<\/jats:italic> is immobilized by entrapment. The xerogel synthesized here, a glass\u2010like material, proved to be very effective in maintaining the enzyme on its structure, with an immobilization yield of 96\u2009% and an activity of 5.3\u2005U\/mg (free lipase=12.1\u2005U\/mg). By grinding this xerogel, the immobilization yield remained constant (94\u2009%). Nevertheless, grinding the xerogel allowed better diffusion of reagents, and the activity reached 9.7\u2005U\/mg. Using this xerogel in biodiesel production had a positive effect on CALB efficiency by increasing the biodiesel yield, 46.1\u2009% against 15.1\u2009% with free CALB. Finally, the catalyst could be reused for five runs without loss of activity.<\/jats:p>","DOI":"10.1002\/cbic.202400327","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T10:19:20Z","timestamp":1734949160000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Entrapment of Lipase from <i>Candida antarctica<\/i> in a Xerogel for the Production of Biodiesel from Waste Cooking Oil"],"prefix":"10.1002","volume":"26","author":[{"given":"C\u00e9dric","family":"Decarpigny","sequence":"first","affiliation":[{"name":"UMR Transfrontali\u00e8re 1158 BioEcoAgro Univ. Lille INRAE Univ. Li\u00e8ge UPJV JUNIA Univ. Artois Univ. Littoral C\u00f4te d'Opale ICV\u2013Institut Charles Viollette  59000 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muriel","family":"Bigan","sequence":"additional","affiliation":[{"name":"UMR Transfrontali\u00e8re 1158 BioEcoAgro Univ. Lille INRAE Univ. Li\u00e8ge UPJV JUNIA Univ. Artois Univ. Littoral C\u00f4te d'Opale ICV\u2013Institut Charles Viollette  59000 Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6007-0754","authenticated-orcid":false,"given":"R\u00e9nato","family":"Froidevaux","sequence":"additional","affiliation":[{"name":"UMR Transfrontali\u00e8re 1158 BioEcoAgro Univ. Lille INRAE Univ. Li\u00e8ge UPJV JUNIA Univ. Artois Univ. 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