{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T17:15:25Z","timestamp":1779038125815,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T00:00:00Z","timestamp":1723161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2024,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Photodynamic therapy (PDT) is a noninvasive approach to cancer treatment, wherein cell death is initiated by singlet oxygen (<jats:sup>1<\/jats:sup>O<jats:sub>2<\/jats:sub>) production via energy transfer from excited photosensitizers to ground\u2010state O<jats:sub>2<\/jats:sub>. Effective clinical photosensitizers necessitate water solubility for in\u2005vivo administration. Hydrophobic dyes, such as phthalocyanines, cannot be used directly as photosensitizers. Herein, we synthesized a myoglobin\u2010(human serum albumin) fusion protein reconstituted with zinc\u2010phthalocyanine (ZnPc), termed ZnPcMb\u2010HSA. The photophysical properties of ZnPcMb\u2010HSA closely resemble those of ZnPc\u2010substituted Mb. Notably, ZnPc dissociates from ZnPcMb\u2010HSA and selectively accumulates within cancer cells, while the protein components remain extracellular. Treatment of four distinct cell lines with ZnPcMb\u2010HSA, followed by red\u2010light irradiation, effectively induced apoptosis. The half\u2010maximal inhibitory concentrations (IC<jats:sub>50<\/jats:sub>) against these cancer cell lines ranged between 0.1\u20130.5\u2005\u03bcM. Reconstituted Mb\u2010HSA emerges as a promising carrier for transporting various water\u2010insoluble porphyrinoid photosensitizer to target cancer cells in PDT applications.<\/jats:p>","DOI":"10.1002\/cbic.202400329","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T22:10:47Z","timestamp":1719439847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Transport of Zinc\u2010Phthalocyanine to Cancer Cells Using Myoglobin\u2212Albumin Fusion Protein for Photodynamic Therapy"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4118-5828","authenticated-orcid":false,"given":"Taiga","family":"Yamada","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Engineering Chuo University  1-13-27 Kasuga Bunkyo-ku Tokyo 112-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mizuki","family":"Funamoto","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Engineering Chuo University  1-13-27 Kasuga Bunkyo-ku Tokyo 112-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoya","family":"Takada","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Engineering Chuo University  1-13-27 Kasuga Bunkyo-ku Tokyo 112-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9288-4840","authenticated-orcid":false,"given":"Yoshitsugu","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Engineering Chuo University  1-13-27 Kasuga Bunkyo-ku Tokyo 112-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7622-3433","authenticated-orcid":false,"given":"Teruyuki","family":"Komatsu","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Faculty of Science and Engineering Chuo University  1-13-27 Kasuga Bunkyo-ku Tokyo 112-8551 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,9]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.3c00633"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1071"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9MD00558G"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.0c00047"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CS00173B"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.7b00274"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dyepig.2020.108455"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.8b00676"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0DT03597A"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.202300373"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b03368"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12274-019-2452-2"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.0c00450"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202203952"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(59)90407-X"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99469-X"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2019.106228"},{"key":"e_1_2_8_18_1","first-page":"s18","volume":"11","author":"Caraceni P.","year":"2013","journal-title":"Blood Transfus."},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012369120376"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.1c00046"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.9b00222"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5666"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/35051594"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-1185"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.9b00373"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2018.02.037"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2022.02.013"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7DT03976J"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202400329","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T06:24:48Z","timestamp":1760336688000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.202400329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,9]]},"references-count":28,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2024,9,2]]}},"alternative-id":["10.1002\/cbic.202400329"],"URL":"https:\/\/doi.org\/10.1002\/cbic.202400329","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,9]]},"assertion":[{"value":"2024-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202400329"}}