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The efficacy of this system was evaluated using an experimental bacteremia model. Endothelial cells exhibited increased ROS\/RNS production when stimulated by <jats:italic>Staphylococcus aureus<\/jats:italic>. However, they remained inactive when exposed to either unprimed or primed neutrophils that had been pre\u2010incubated with bacteria. It is noteworthy that the sequential stimulation of endothelial cells with bacteria followed by neutrophils resulted in a significant increase in the ROS\/RNS level, which demonstrated a correlation with the number of neutrophils in contact with the endothelial cells. These results highlight the potential of our system to quantitatively assess ROS\/RNS dynamics in complex biological systems. 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