{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:54:43Z","timestamp":1760316883297,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004505","name":"Universit\u00e0 di Catania","doi-asserted-by":"publisher","award":["2018\/2020","2020\/2022"],"award-info":[{"award-number":["2018\/2020","2020\/2022"]}],"id":[{"id":"10.13039\/501100004505","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2024,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report a water\u2010soluble fluorescence and colorimetric copper probe (<jats:bold>LysoBC1<\/jats:bold>); this system can also serve for lysosome labeling and for the dynamic tracking of Cu<jats:sup>2+<\/jats:sup> in living cells. The sensing mechanism takes advantage of the synergic action by the following three components: i)\u2005a lysosome targeting unit, ii)\u2005the spirolactam ring\u2010opening for the selective copper chelation and iii)\u2005the metal\u2010mediated hydrolysis of the rhodamine moiety for fluorescence enhancement. In aqueous environment the molecule acts as a fluorescent reversible pH sensor and as colorimetric probe for Cu<jats:sup>2+<\/jats:sup> at physiological pH; the hydrolysis of the copper targeting unit resulted in a 50\u2010fold increase of the fluorescence intensity. Most importantly, in\u2005vitro cell analyses in undifferentiated (SH SY5Y) and differentiated (d\u2010SH SY5Y) neuroblastoma cells, <jats:bold>LysoBC1<\/jats:bold> is able to selectively accumulate into lysosome while the copper binding ability allowed us to monitor intracellular copper accumulation into lysosome.<\/jats:p>","DOI":"10.1002\/cbic.202400377","type":"journal-article","created":{"date-parts":[[2024,7,28]],"date-time":"2024-07-28T22:18:27Z","timestamp":1722205107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Water\u2010Soluble Multifunctional Probe for Colorimetric Copper Sensing, Lysosome Labelling and Live\u2010Cell Imaging"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9112-5623","authenticated-orcid":false,"given":"Carmela","family":"Bonaccorso","sequence":"first","affiliation":[{"name":"Laboratory of Molecular modelling and Heterocyclic compounds (ModHet) Department of Chemical Sciences University of Catania  Viale Andrea Doria n.\u20056 95125 Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena","family":"Maria Cucci","sequence":"additional","affiliation":[{"name":"Laboratory of Hybrid NanoBioInterfaces (NHBIL) Department of Chemical Sciences University of Catania  Viale Andrea Doria n.\u20056 95125 Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Sanfilippo","sequence":"additional","affiliation":[{"name":"Laboratory of Hybrid NanoBioInterfaces (NHBIL) Department of Chemical Sciences University of Catania  Viale Andrea Doria n.\u20056 95125 Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Munzone","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular modelling and Heterocyclic compounds (ModHet) Department of Chemical Sciences University of Catania  Viale Andrea Doria n.\u20056 95125 Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1889-886X","authenticated-orcid":false,"given":"Cosimo G.","family":"Fortuna","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular modelling and Heterocyclic compounds (ModHet) Department of Chemical Sciences University of Catania  Viale Andrea Doria n.\u20056 95125 Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Satriano","sequence":"additional","affiliation":[{"name":"Laboratory of Hybrid NanoBioInterfaces (NHBIL) Department of Chemical Sciences University of Catania  Viale Andrea Doria n.\u20056 95125 Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,12]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.12.001"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-28957-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R114.592618"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20807"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-7372(03)00106-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3565"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6MT00058D"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.04.033"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8091080"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b05550"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9mt00223e"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100910"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.631283"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.1999.0250"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0mt00074d"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1007\/s00775-019-01731-9","volume":"24","author":"Acevedo K.","year":"2019","journal-title":"JBIC Journal of Biological Inorganic Chemistry 2019 24\u2009:\u20098"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0277-5387(94)00423-C"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2018.01.019"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph13010004"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200800005"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja971221g"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b02459"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/1061186X.2017.1374390"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2022.214695"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.inorgchem.7b02720"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo070246n"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(02)00120-0"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0277-5387(99)00310-1"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3934\/biophy.2015.3.336"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CS00719H"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CS00083G"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.26346"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00710-4"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-012-9311-1"},{"key":"e_1_2_8_35_1","first-page":"1","volume":"12","author":"Xicoy H.","year":"2017","journal-title":"Mol. Neurodegener. 2017 12\u2009:\u20091"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159051"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja4057807"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202400377","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:29:21Z","timestamp":1760297361000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.202400377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,12]]},"references-count":37,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2024,11,4]]}},"alternative-id":["10.1002\/cbic.202400377"],"URL":"https:\/\/doi.org\/10.1002\/cbic.202400377","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"type":"print","value":"1439-4227"},{"type":"electronic","value":"1439-7633"}],"subject":[],"published":{"date-parts":[[2024,9,12]]},"assertion":[{"value":"2024-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202400377"}}