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This serotype contains an <jats:italic>O<\/jats:italic>\u2010acetyl group at the internal L\u2010rhamnose of its polysaccharide repeating unit. Herein we report on the role of the <jats:italic>O<\/jats:italic>\u2010acetyl moiety of 7F polysaccharide in both antigen recognition and the induction of a protective antibody response against 7F. Fully and partially de\u2010<jats:italic>O<\/jats:italic>\u2010acetylated 7F polysaccharides were chemically prepared and compared with the <jats:italic>O<\/jats:italic>\u2010acetylated counterpart in their antigenicity and immunogenicity of their tetanus toxoid glycoconjugates. These comparative studies showed a slight but consistent decrease in the antigenicity for the fully de\u2010<jats:italic>O<\/jats:italic>\u2010acetylated polysaccharide, but not for the partly de\u2010<jats:italic>O<\/jats:italic>\u2010acetylated variant. The glycoconjugates derived from the <jats:italic>O<\/jats:italic>\u2010acetylated and the fully de\u2010<jats:italic>O<\/jats:italic>\u2010acetylated polysaccharides had similar sizes and polysaccharide\u2010to\u2010protein ratio, and all proved both to be immunogenic and induce opsonophagocytic responses in mice. Nevertheless, the immune response elicited by the <jats:italic>O<\/jats:italic>\u2010acetylated glycoconjugate was better in both quantity and quality, proving that the <jats:italic>O<\/jats:italic>\u2010acetyl group is not strictly necessary but also not irrelevant for the antigenicity and immunogenicity of the 7F serotype polysaccharide and its glycoconjugates.<\/jats:p>","DOI":"10.1002\/cbic.202400684","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T03:00:21Z","timestamp":1728010821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of <i>O<\/i>\u2010Acetylation on the Antigenicity and Glycoconjugate Immunogenicity of the <i>Streptococcus Pneumoniae<\/i> Serotype 7F Capsular Polysaccharide"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9097-302X","authenticated-orcid":false,"given":"Jean\u2010Pierre","family":"Soubal","sequence":"first","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aloyma","family":"Lugo","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5333-554X","authenticated-orcid":false,"given":"Darielys","family":"Santana\u2010Mederos","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raine","family":"Garrido","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura M.","family":"Rodriguez\u2010Noda","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rocmira","family":"Perez\u2010Nicado","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yamilka","family":"Soroa\u2010Millan","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mildrey","family":"Fari\u00f1as","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0638-3896","authenticated-orcid":false,"given":"Yury","family":"Vald\u00e9s\u2010Balb\u00edn","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2099-1791","authenticated-orcid":false,"given":"Dagmar","family":"Garc\u00eda\u2010Rivera","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5538-1555","authenticated-orcid":false,"given":"Daniel G.","family":"Rivera","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"},{"name":"Laboratory of Synthetic and Biomolecular Chemistry Faculty of Chemistry University of Havana, Zapata &amp; G  Havana 10400 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5596-6847","authenticated-orcid":false,"given":"Vicente","family":"V\u00e9rez\u2010Bencomo","sequence":"additional","affiliation":[{"name":"Finlay Institute of Vaccines  200 and 21 Street Havana 11600 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,6]]},"reference":[{"key":"e_1_2_8_1_1","unstructured":"\u00a0"},{"key":"e_1_2_8_2_2","doi-asserted-by":"crossref","unstructured":"K.\u2005A. 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