{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:00:17Z","timestamp":1790787617954,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T00:00:00Z","timestamp":1730160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2025,2,16]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Imine reductases (IREDs) provide promising opportunities for the synthesis of various chiral amines. Initially, asymmetric imine reduction was reported, followed by reductive aminations of aldehydes and ketones <jats:italic>via<\/jats:italic> imines. Herein we present the reductive amination of structurally diverse carbonyls and dicarbonyls with hydrazines (reductive hydrazination), catalyzed by the IRED from <jats:italic>Myxococcus stipitatus<\/jats:italic>. In analogy to IRED\u2010catalyzed reductive aminations, various carbonyls and dicarbonyls could react with simple hydrazines to produce substituted acyclic and cyclic <jats:italic>N<\/jats:italic>\u2010alkylhydrazines. By incorporating and scaling up a hydrogenase cofactor regeneration system, we demonstrated the scalability and atom\u2010efficiency of an H<jats:sub>2<\/jats:sub>\u2010driven double reductive hydrazination, highlightling the potential of IREDs in biocatalysis.<\/jats:p>","DOI":"10.1002\/cbic.202400700","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T04:12:19Z","timestamp":1725941539000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Synthesis of Substituted Acyclic and Cyclic <i>N<\/i>\u2010Alkylhydrazines by Enzymatic Reductive Hydrazinations"],"prefix":"10.1002","volume":"26","author":[{"given":"Niels","family":"Borlinghaus","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Technical Biochemistry Department of Technical Biochemistry Universitaet Stuttgart  Allmandring 31 70569 Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donato","family":"Calabrese","sequence":"additional","affiliation":[{"name":"Institute of Applied Microbiology (iAMB) RWTH Aachen University  Worringer weg 1 52074 Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6601-6473","authenticated-orcid":false,"given":"Lars","family":"Lauterbach","sequence":"additional","affiliation":[{"name":"Institute of Applied Microbiology (iAMB) RWTH Aachen University  Worringer weg 1 52074 Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1282-195X","authenticated-orcid":false,"given":"Bettina M.","family":"Nestl","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Technical Biochemistry Department of Technical Biochemistry Universitaet Stuttgart  Allmandring 31 70569 Stuttgart Germany"},{"name":"Innophore GmbH  Am Eisernen Tor 3 8010 Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,29]]},"reference":[{"key":"e_1_2_3_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200300599"},{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201500764"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201600384"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2782"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201402218"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6GC03023H"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-019-0249-z"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201402625"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/b010091a"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.2514\/1.B35086"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0504409"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.4c01119"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2014.10.011"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/np400124n"},{"key":"e_1_2_3_15_1","doi-asserted-by":"crossref","unstructured":"D. Van\u2005Cura T.\u2005L. Ng J. Huang H. Hager J.\u2005F. Hartwig J.\u2005D. Keasling E. Balskus Angew. Chemie - Int. Ed.2023 62 e202304646.","DOI":"10.1002\/anie.202304646"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2550110403"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(77)80001-3"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201600647"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.8b00291"},{"key":"e_1_2_3_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8GC03798A"},{"key":"e_1_2_3_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202001302"},{"key":"e_1_2_3_22_1","first-page":"4853","volume":"12","author":"Preissler J.","year":"2020","journal-title":"Angew. Chemie - Int. 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