{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:37:06Z","timestamp":1790883426995,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T00:00:00Z","timestamp":1730851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018934","name":"Universit\u00e4t Greifswald","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018934","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BO 1862\/23-1"],"award-info":[{"award-number":["BO 1862\/23-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2025,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Esters are valuable aroma compounds and can be produced enzymatically by Baeyer\u2010Villiger monooxygenases (BVMOs) from (aliphatic) ketone precursors. However, a genetically encoded biosensor system for the assessment of BVMO activity and the detection of reaction products is missing. In this work, we assembled a synthetic enzyme cascade \u2013 featuring an esterase, an alcohol dehydrogenase, and LuxAB \u2013 in the heterologous host <jats:italic>Escherichia coli<\/jats:italic>. Target esters are produced by a BVMO, subsequently cleaved, and the corresponding alcohol oxidized through the artificial pathway. Ultimately, aldehyde products are detected <jats:italic>in\u2005vivo<\/jats:italic> by LuxAB, a luciferase from <jats:italic>Photorhabdus luminescens<\/jats:italic> that emits bioluminescence upon the oxidation of aldehydes to the corresponding carboxylates. This biosensor system greatly accelerated the screening and selection of active BVMO variants from a focused library, omitting commonly used low\u2010throughput chromatographic analysis. Engineered enzymes accepted linear aliphatic ketones such as 2\u2010undecanone and 2\u2010dodecanone and exhibited improved ester formation.<\/jats:p>","DOI":"10.1002\/cbic.202400712","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T11:55:39Z","timestamp":1727265339000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Biosensor\u2010Guided Engineering of a Baeyer\u2010Villiger Monooxygenase for Aliphatic Ester Production"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3138-9357","authenticated-orcid":false,"given":"Thaleia","family":"Sakoleva","sequence":"first","affiliation":[{"name":"Department of Biotechnology &amp; Enzyme Catalysis Institute of Biochemistry University of Greifswald  Felix-Hausdorff-Str. 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8005-9283","authenticated-orcid":false,"given":"Florian","family":"Vesenmaier","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Enzyme Catalysis Institute of Biochemistry University of Greifswald  Felix-Hausdorff-Str. 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Enzyme Catalysis Institute of Biochemistry University of Greifswald  Felix-Hausdorff-Str. 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarne E.","family":"Schunke","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Enzyme Catalysis Institute of Biochemistry University of Greifswald  Felix-Hausdorff-Str. 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kay D.","family":"Novak","sequence":"additional","affiliation":[{"name":"acib GmbH  Krenngasse 37\/2 8010 Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha","family":"Grobe","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Enzyme Catalysis Institute of Biochemistry University of Greifswald  Felix-Hausdorff-Str. 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3270-6895","authenticated-orcid":false,"given":"Mark","family":"D\u00f6rr","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Enzyme Catalysis Institute of Biochemistry University of Greifswald  Felix-Hausdorff-Str. 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0685-2696","authenticated-orcid":false,"given":"Uwe T.","family":"Bornscheuer","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Enzyme Catalysis Institute of Biochemistry University of Greifswald  Felix-Hausdorff-Str. 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0656-3280","authenticated-orcid":false,"given":"Thomas","family":"Bayer","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Enzyme Catalysis Institute of Biochemistry University of Greifswald  Felix-Hausdorff-Str. 4 17487 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,6]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11117"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adh8615"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CS01575J"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202006648"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b03396"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CC00368D"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2012.05.098"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.201500202"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202200746"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2013.11.003"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcat.2020.110875"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr1003437"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8NP00054A"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.200700045"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a022494"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2017.09.008"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2017.11.007"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201600484"},{"key":"e_1_2_8_19_1","first-page":"2484","volume":"54","author":"Schmidt S.","year":"2015","journal-title":"Angew. Chem. Int. Ed."},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201300957"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.2.1127"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8636-3"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/1087057113480390"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/catal11080953"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CS00981C"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202200228"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.200505040"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2020.03.013"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c01741"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2015.06.008"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-018-0869-z"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104326"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/synbio\/ysz028"},{"key":"e_1_2_8_34_1","doi-asserted-by":"crossref","unstructured":"B.\u2005M. Woolston T. Roth I. Kohale D.\u2005R. Liu G. Stephanopoulos Biotechnol. & Bioeng.2018 115 206\u2013215.","DOI":"10.1002\/bit.26455"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.5b00069"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/sb300091d"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.slast.2023.10.005"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnx053"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409523111"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3595"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18813-x"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13383"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2013.06.061"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.10.2601-2606.1989"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja506664a"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03319-14"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-11-90"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.200600174"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201700464"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1288"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1061"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7366-8_4"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gnh110"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.2.488"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404538101"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202404492"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5331.1453"},{"key":"e_1_2_8_59_1","first-page":"231","volume-title":"The Enzymes","author":"Schmidt S.","year":"2020"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202216962"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.01.016"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.12.054"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202400712","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T17:26:44Z","timestamp":1760290004000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.202400712"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,6]]},"references-count":62,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1,2]]}},"alternative-id":["10.1002\/cbic.202400712"],"URL":"https:\/\/doi.org\/10.1002\/cbic.202400712","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,6]]},"assertion":[{"value":"2024-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202400712"}}