{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T23:41:32Z","timestamp":1760312492855,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["P20222272N_01","P20222272N_02"],"award-info":[{"award-number":["P20222272N_01","P20222272N_02"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2025,4,14]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we employed a novel fluorescent probe, RO7304924 \u2013 which selectively targets cannabinoid 2 receptor (CB<jats:sub>2<\/jats:sub>R) \u2013 to assess the lateral mobility of CB<jats:sub>2<\/jats:sub>R within the plasma membrane of Chinese hamster ovary cells stably expressing a functional, untagged receptor variant. Utilizing confocal fluorescence recovery after photobleaching (FRAP), we quantified the diffusion coefficient and mobile fraction of CB<jats:sub>2<\/jats:sub>R, thereby demonstrating the efficacy of RO7304924 as an innovative tool for elucidating the dynamics of this major endocannabinoid\u2010binding G protein\u2010coupled receptor. Our present findings highlight the potential of combining advanced ligand\u2010based fluorescent probes with FRAP for future investigations into the biochemical details of CB<jats:sub>2<\/jats:sub>R mobility in living cells, and its impact on receptor\u2010dependent cellular processes.<\/jats:p>","DOI":"10.1002\/cbic.202400921","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T05:41:07Z","timestamp":1737006067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Probing Native CB<sub>2<\/sub> Receptor Mobility in Plasma Membranes of Living Cells by Fluorescence Recovery After Photobleaching"],"prefix":"10.1002","volume":"26","author":[{"given":"Francesca","family":"Ciaramellano","sequence":"first","affiliation":[{"name":"European Center for Brain Research\/Institute for Research and Health Care (IRCCS) Santa Lucia Foundation  Via del Fosso di Fiorano 64 00143 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Leuti","sequence":"additional","affiliation":[{"name":"European Center for Brain Research\/Institute for Research and Health Care (IRCCS) Santa Lucia Foundation  Via del Fosso di Fiorano 64 00143 Rome Italy"},{"name":"Department of Medicine Campus Bio-Medico University of Rome  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandrine D. E.","family":"Kurtz","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Technology for Food Agriculture and Environment University of Teramo  64100 Teramo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"Sarott","sequence":"additional","affiliation":[{"name":"Laboratorium f\u00fcr Organische Chemie ETH Zurich  Vladimir-Prelog-Weg 3 Zurich CH-8093 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Westphal","sequence":"additional","affiliation":[{"name":"Laboratorium f\u00fcr Organische Chemie ETH Zurich  Vladimir-Prelog-Weg 3 Zurich CH-8093 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Pfaff","sequence":"additional","affiliation":[{"name":"Laboratorium f\u00fcr Organische Chemie ETH Zurich  Vladimir-Prelog-Weg 3 Zurich CH-8093 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"Grether","sequence":"additional","affiliation":[{"name":"Pharma Research and Early Development (pRED) Roche Innovation Center Basel F. Hoffmann-La Roche Ltd  Grenzacherstrasse 124 Basel CH-4070 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erick M.","family":"Carreira","sequence":"additional","affiliation":[{"name":"Laboratorium f\u00fcr Organische Chemie ETH Zurich  Vladimir-Prelog-Weg 3 Zurich CH-8093 Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Maccarrone","sequence":"additional","affiliation":[{"name":"European Center for Brain Research\/Institute for Research and Health Care (IRCCS) Santa Lucia Foundation  Via del Fosso di Fiorano 64 00143 Rome Italy"},{"name":"Department of Biotechnological and Applied Clinical Sciences University of L'Aquila  Via Vetoio snc 67100 L'Aquila Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6217-698X","authenticated-orcid":false,"given":"Sergio","family":"Oddi","sequence":"additional","affiliation":[{"name":"European Center for Brain Research\/Institute for Research and Health Care (IRCCS) Santa Lucia Foundation  Via del Fosso di Fiorano 64 00143 Rome Italy"},{"name":"Department of Veterinary Medicine University of Teramo  via Renato Balzarini 1 64100 Teramo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,12]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202301325R"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.54.2.161"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2008.01671.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19030833"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-21737-2_1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1124\/pharmrev.122.000600"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11071237"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.10.5.653"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2017.02.023"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-019-0284-z"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.07.021"},{"key":"e_1_2_9_12_1","first-page":"30","volume":"11","author":"Cassano T.","year":"2017","journal-title":"Front. Neurol. Neurosci."},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-020-00530-2"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/164_2017_57"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.09.001"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25204729"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986711797379285"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1478-811X-7-16"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.0c05587"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1SC06659E"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)51566-0"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01658.x"},{"key":"e_1_2_9_23_1","first-page":"Unit2.19","volume":"2","author":"Day C. A.","year":"2012","journal-title":"Curr. Protoc. Cytom."},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(83)84410-5"},{"key":"e_1_2_9_25_1","unstructured":"H. Wickham ggplot22009 VIII 213."},{"key":"e_1_2_9_26_1","unstructured":"H. Bengtsson \u201cFunctions that Apply to Rows and Columns of Matrices (and to Vectors) [R package matrixStats version 1.3.0] \u201d can be found underhttps:\/\/cran.r-project.org\/package=matrixStats 2024."},{"key":"e_1_2_9_27_1","unstructured":"M. Maechler DOIhttp:\/\/specfun.r-forge.r-project.org 2022."},{"key":"e_1_2_9_28_1","unstructured":"A. Kassambara \u201c\u2018ggplot2\u2019 Based Publication Ready Plots [R package ggpubr version 0.6.0] \u201d can be found underhttps:\/\/cran.r-project.org\/package=ggpubr 2023."},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1304"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0185"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.51.2.177"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.076695"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2019.04.012"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.6b00517"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp106981c"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00188040"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1559-4106-8-1"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcc.6b07875"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202400921","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T22:58:17Z","timestamp":1760309897000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.202400921"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,12]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,4,14]]}},"alternative-id":["10.1002\/cbic.202400921"],"URL":"https:\/\/doi.org\/10.1002\/cbic.202400921","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"type":"print","value":"1439-4227"},{"type":"electronic","value":"1439-7633"}],"subject":[],"published":{"date-parts":[[2025,3,12]]},"assertion":[{"value":"2024-11-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202400921"}}