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Used as a carrier protein in conjugate vaccines, it is crucial to preserve their self\u2010adjuvant properties during the conjugation step. Considering the absence of cysteine in the <jats:italic>Salmonella enterica<\/jats:italic> flagellin FliC sequence, we have investigated the impact of five mutations (A2\u2005C, K180\u2005C, T240\u2005C, D251\u2005C and S306\u2005C) alone or in combination on TLR5 activation. The FliC mutated at the four positions K180\u2005C, T240\u2005C, D251\u2005C and S306\u2005C displayed much the same activity as native flagellin whether the cysteine residues were free or conjugated. 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