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Biophysical techniques have revolutionized this field over time, providing remarkable insights into structure\u2010function relationships. Among these, Site\u2010Directed Spin Labelling (SDSL) combined with Electron Paramagnetic Resonance (EPR) is a powerful method delivering structural data at the residue level, irrespective of protein size or environment. Traditional nitroxide labels targeting cysteine residues face limitations when these residues are essential for protein structure or function. To address this, alternatives have been proposed as the use of non\u2010canonical amino acids (ncaa) coupled with specific nitroxide labels. This study introduces\n                    <jats:sup>14<\/jats:sup>\n                    N\u2010HO\u20105223, a novel nitroxide label specific to the\n                    <jats:italic>p<\/jats:italic>\n                    AzPhe ncaa, along with its\n                    <jats:sup>15<\/jats:sup>\n                    N\u2010derivative. These labels were grafted at two sites of the model protein, the diflavin cytochrome P450 reductase. For comparative purpose, two already reported labels were also used. Continuous wave (cw) EPR spectroscopy confirmed the HO\u20105223 label as an effective reporter of protein dynamics. Additionally, Double Electron\u2010Electron Resonance (DEER) measurements provided distance distributions between the semi\u2010quinone FMNH\u22c5 state of the CPR and all nitroxide labels. These results expand the toolkit of the ncaa\u2010nitroxide pairs, enabling EPR\u2010based structural studies of proteins where cysteine modification is impractical, further advancing our ability to decode protein dynamics and function.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500064","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T09:27:43Z","timestamp":1741685263000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Expanding the Diversity of Nitroxide\u2010Based Paramagnetic Probes Conjugated to Non\u2010Canonical Amino Acids for Sdsl\u2010Epr Applications"],"prefix":"10.1002","volume":"26","author":[{"given":"Maxime","family":"Bizet","sequence":"first","affiliation":[{"name":"Aix Marseille Univ CNRS BIP  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ron","family":"Bal\u00e1zsi","sequence":"additional","affiliation":[{"name":"Institute of Organic and Medicinal Chemistry Faculty of Pharmacy University of P\u00e9cs  Honv\u00e9d st.\u20051. 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