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This study characterizes these microbubbles using high\u2010speed imaging. Microbubbles generated within cells by single\u2010pulse irradiation are consistently larger than those formed in the surrounding culture medium across a broad pulse energy range. Notably, the energy threshold for intracellular microbubble formation is several tens of nanojoules lower than in the culture medium, enabling precise cell ablation without inducing extracellular bubbling. Repeated pulse irradiation at 1\u2009kHz frequency further reduces the energy threshold and increases bubble size, with saturation observed after \u224830 pulses. Near\u2010threshold pulse irradiation is then employed to develop a precise method for investigating cellular motility in\u2009vitro. Cell\u2010free zones are created within cell monolayers, enabling motile cells to migrate into the cleared zones. This method is validated using the A549 lung cancer cell line and the plant flavonoid apigenin, which exhibits dose\u2010dependent reduction in A549 cell motility. 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