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Various metabolites that accumulate in IEMs have been reported to self\u2010assemble into organized structures. These structures exhibit similar physicochemical properties as proteinaceous amyloid fibrils. Our study illustrates the aggregation properties of branched chain amino acids (BCAAs), isoleucine, leucine, and valine that accumulate in maple syrup urine disease (MSUD) to investigate their propensities to assemble into amyloid\u2010like fibrils. The structural morphologies of BCAAs are studied via microscopic techniques. Further, characterization techniques are employed to understand the physicochemical properties of the self\u2010assemblies and their underlying mechanism. The amyloid\u2010like nature of these aggregates is confirmed using thioflavin T and congo red assays. The (3\u2010(4,5\u2010Dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide) assay reveals BCAAs are cytotoxic and significantly decrease cell viability. 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