{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:37:16Z","timestamp":1792615036616,"version":"4.5.0"},"reference-count":53,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2025,11,17]]},"abstract":"<jats:p>\n                    Properties of semisynthetic hydrogels can be fine\u2010tuned, making these attractive for various applications in regenerative medicine. Herein, a hydrogel platform based on hyaluronic acid (HA) modified by (1R, 8S,9S)\u2010bicycle[6.1.0]non\u20104\u2010yn\u20109\u2010ylmethanol (BCN) and a cross\u2010linker composed of light\u2010sensitive\n                    <jats:italic>o<\/jats:italic>\n                    \u2010nitrobenzyl and polyethylene glycol (PEG) chains terminating in azides is described. The two components can undergo strain\u2010promoted azide\u2013alkyne cycloaddition resulting in rapid gel formation. First, adipose\u2010derived mesenchymal stromal cells (MSCs) are incorporated in the hydrogel and it is demonstrated that the cells can be easily retrieved by UV light\u2010mediated degradation, maintaining viability and retaining spindle\u2010like shape when the cells are replated. Next, a proof\u2010of\u2010concept of inducing light\u2010mediated softening of the hydrogel to modulate the morphology of the encapsulated cells is provided. A co\u2010culture of endothelial cells (cord blood\u2010derived endothelial colony forming cells and bone marrow\u2010derived MSCs), which is commonly studied for their ability to form capillary\u2010like vascular networks, is cultured in the regular and light\u2010induced softened hydrogels. Nonphotoexposed hydrogels show cells with a prevalently rounded morphology, whereas stretched cells connecting into a primitive capillary network are observed in the light\u2010softened hydrogels. Photo\u2010induced softening offers potential to locally control cell shape and self\u2010organization capacity.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500310","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T11:03:43Z","timestamp":1751454223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Photo\u2010Triggered Hyaluronic Acid Hydrogel for 3D Culture and Cell Recovery"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0183-3384","authenticated-orcid":false,"given":"Francesco","family":"Palmieri","sequence":"first","affiliation":[{"name":"Division of Chemical Biology and Drug Discovery Utrecht Institute for Pharmaceutical Sciences Utrecht University  3584 CG Utrecht 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