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Deficiency in frataxin is associated with Friedreich's ataxia, a progressive neurodegenerative disorder. CyaY, the bacterial ortholog of eukaryotic frataxin, is believed to function as an iron donor in Fe\u2013S cluster assembly, making it a key target for structural and functional studies. In this work, a comprehensive structural analysis of the\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    CyaY protein is presented, comparing its structure at room temperature and cryogenic conditions. Notably, the first room\u2010temperature structures are obtained using the Turkish Light Source \u201cTurkish DeLight\u201d X\u2010ray diffractometer and serial synchrotron X\u2010ray crystallography, marking a significant step forward in understanding CyaY under near\u2010physiological conditions.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500442","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T13:43:24Z","timestamp":1761659004000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative Structural Analysis of Escherichia Coli Cyay at Room and Cryogenic Temperatures Using Macromolecular and Serial Crystallography"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2424-9583","authenticated-orcid":false,"given":"Alaleh","family":"Shafiei","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics Koc University  34450 Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8829-3287","authenticated-orcid":false,"given":"Nilufer","family":"Baldir","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Koc University  34450 Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jongbum","family":"Na","sequence":"additional","affiliation":[{"name":"Department of New Biology Daegu Gyeongbuk Institute of Science and Technology (DGIST)  Daegu 42988 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Hae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of New Biology Daegu Gyeongbuk Institute of Science and Technology (DGIST)  Daegu 42988 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9135-5397","authenticated-orcid":false,"given":"Hasan","family":"DeMirci","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Koc University  34450 Istanbul Turkey"},{"name":"SLAC National Laboratory Stanford PULSE Institute  Menlo Park 94025 CA USA"},{"name":"Koc University Isbank Center for Infectious Diseases (KUISCID) Koc University  34010 Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5326.653"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.74.082803.133518"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2014.11.018"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007750050002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio0406-171"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08301"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.05.011"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi061237w"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi801565j"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2005.01.006"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403721200"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(96)20054-2"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M513569200"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000354"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.160270897"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.1c00112"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00690-9"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308315101"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583520000128"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1111325108"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1302823110"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1323440111"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798318010240"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1954"},{"key":"e_1_2_9_26_1","unstructured":"C. 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