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However, the regulatory factors in A\u03b2 metabolism remain poorly understood. Herein, three homogeneously glycosylated A\u03b2\n                    <jats:sub>42<\/jats:sub>\n                    peptides are utilized to explore the impacts of Tyr10\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycosylation on A\u03b2 clearance in astrocytes. Based on various biochemical and cellular assays, it is shown that the introduced \u03b1\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycan stabilizes the A\u03b2 oligomers and enhances A\u03b2\n                    <jats:sub>42<\/jats:sub>\n                    endocytosis and autophagy in astrocytes, which ultimately promotes the intracellular degradation of A\u03b2\n                    <jats:sub>42<\/jats:sub>\n                    and the secretion of A\u03b2\u2010degrading enzymes. Particularly, a disaccharide, Gal\u03b21\u20103GalNAc, exhibits the most substantial clearance\u2010enhancing effect. Moreover, experiments with AD\u2010like model mice show protective effects from the disaccharide modification in alleviating A\u03b2\n                    <jats:sub>42<\/jats:sub>\n                    \u2010induced impairment of spatial cognitive performance. Thus, beyond showing the influences induced by particular\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycosylation on A\u03b2\n                    <jats:sub>42<\/jats:sub>\n                    degradation, the study provides implications of a possible role of Tyr10\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycan in regulating A\u03b2 clearance in the brain.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500459","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T10:37:08Z","timestamp":1764585428000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["\u03b1\u2010\n                    <i>O<\/i>\n                    \u2010Glycosylation at Tyrosine 10 Promotes the Astrocyte Clearance of Amyloid\u2010\u03b2 Peptide 1\u201342"],"prefix":"10.1002","volume":"27","author":[{"given":"Lu","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs Chemical Biology Center and Department of Chemical Biology at School of Pharmaceutical Sciences Peking University  Beijing 100191 China"},{"name":"Ningbo Institute of Marine Medicine Peking University  Ningbo 315832 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dangliang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs Chemical Biology Center and Department of Chemical Biology at School of Pharmaceutical Sciences Peking University  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qijia","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs Chemical Biology Center and Department of Chemical Biology at School of Pharmaceutical Sciences Peking University  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changdong","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs Chemical Biology Center and Department of Chemical Biology at School of Pharmaceutical Sciences Peking University  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs Chemical Biology Center and Department of Chemical Biology at School of Pharmaceutical Sciences Peking University  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2648-4344","authenticated-orcid":false,"given":"Suwei","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs Chemical Biology Center and Department of Chemical Biology at School of Pharmaceutical Sciences Peking University  Beijing 100191 China"},{"name":"Ningbo Institute of Marine Medicine Peking University  Ningbo 315832 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,1]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-061010-113613"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3505"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19323"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2018.109"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2757-z"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2985-10.2010"},{"key":"e_1_2_8_8_1","first-page":"1015","volume":"93","author":"Menzies F. 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