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Although RNA molecules can modulate the FUS phase behavior, identifying highly effective sequences remains challenging because of FUS's multiple low\u2010specificity RNA\u2010binding domains. In this study, we rationally designed a 65\u2010mer RNA, U1\u2032+TERRA, by combining a stem\u2010loop\u2013GGU motif and a G\u2010quadruplex (G4) structure, each known to interact with distinct FUS domains. U1\u2032+TERRA exhibited strong binding affinity and effectively inhibited FUS aggregation in vitro. We introduced 2\u2032\u2010O\u2010methyl modifications, generating (U1\u2032+TERRA)\u20102\u2032\u2010OMe, which retained structural integrity and demonstrated resistance to nuclease degradation to enhance biological stability. Notably, (U1\u2032+TERRA)\u20102\u2032\u2010OMe suppressed FUS aggregation even at a low concentration. These findings suggested that multivalent RNA constructs with rationally arranged motifs can serve as potent inhibitors of FUS aggregation. 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