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An excited\u2010state intramolecular proton transfer mechanism (ESIPT)\u2010based reactive probe HBT\u2010EPI has been successfully developed for ratiometric fluorescent detection of EPI in physiological conditions. EPI undergoes a cascade of nucleophilic attack at the carbonothioate site of the probe using the secondary amine and\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010hydroxyl group to release the ESIPT\u2010active 2\u2010(2\u2032\u2010hydroxyphenyl) benzothiazole (HBT) fluorophore. Thus, a ratiometric fluorescence response is obtained. The probe is highly selective for EPI detection compared to other catecholamine\u2010based neurotransmitters. A minimum of 1\u2009\u00b5M of EPI can be easily detected using this probe. HBT\u2010EPI has been successfully demonstrated for ratiometric fluorescence detection of EPI in live cells via confocal imaging.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500631","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T10:13:58Z","timestamp":1762510438000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An Excited\u2010State Intramolecular Proton Transfer Mechanism\u2010Based Reactive Probe for Ratiometric Fluorescence Detection of Epinephrine in Live Cells"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4890-1842","authenticated-orcid":false,"given":"Sk Rajibul","family":"Haque","sequence":"first","affiliation":[{"name":"Department of Natural Products and Medicinal Chemistry CSIR\u2010Indian Institute of Chemical Technology (CSIR\u2010IICT)  Hyderabad 500007 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